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Title

Phenotypic profile of expanded NK cells in chronic lymphoproliferative disorders: a surrogate marker for NK-cell clonality

AuthorsBárcena, Paloma; Jara-Acevedo, Maria; Tabernero, María D.; López, Antonio; Sánchez, Maria Luz; García-Montero, Andrés ; Muñoz-García, Noemí; Vidriales, Maria Belén; Paiva, Artur; Lécrevisse, Quentin; Lima, Margarida; Langerak, Anton W.; Böttcher, Sebastian; Dongen, J. J. M. van; Orfao, Alberto ; Almeida, Julia
KeywordsNatural killer cells
Clonality
Immunophenotype
CLPD-NK
NK cells
Issue Date2015
PublisherImpact Journals
CitationOncotarget 6(40): 42938-42951 (2015)
AbstractCurrently, the lack of a universal and specific marker of clonality hampers the diagnosis and classification of chronic expansions of natural killer (NK) cells. Here we investigated the utility of flow cytometric detection of aberrant/altered NK-cell phenotypes as a surrogate marker for clonality, in the diagnostic work-up of chronic lymphoproliferative disorders of NK cells (CLPD-NK). For this purpose, a large panel of markers was evaluated by multiparametric flow cytometry on peripheral blood (PB) CD56 NK cells from 60 patients, including 23 subjects with predefined clonal (n = 9) and polyclonal (n = 14) CD56 NK-cell expansions, and 37 with CLPD-NK of undetermined clonality; also, PB samples from 10 healthy adults were included. Clonality was established using the human androgen receptor (HUMARA) assay. Clonal NK cells were found to show decreased expression of CD7, CD11b and CD38, and higher CD2, CD94 and HLADR levels vs. normal NK cells, together with a restricted repertoire of expression of the CD158a, CD158b and CD161 killer-associated receptors. In turn, NK cells from both clonal and polyclonal CLPD-NK showed similar/overlapping phenotypic profiles, except for high and more homogeneous expression of CD94 and HLADR, which was restricted to clonal CLPD-NK. We conclude that the CD94/HLADR phenotypic profile proved to be a useful surrogate marker for NK-cell clonality.
DescriptionThis is an open-access article distributed under the terms of the Creative Commons Attribution License.
Publisher version (URL)http://dx.doi.org/10.18632/oncotarget.5480
URIhttp://hdl.handle.net/10261/135729
DOI10.18632/oncotarget.5480
Identifiersdoi: 10.18632/oncotarget.5480
e-issn: 1949-2553
Appears in Collections:(IBMCC) Artículos
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