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http://hdl.handle.net/10261/135416
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Sancho, David | es_ES |
dc.contributor.author | Nieto, Marta | es_ES |
dc.contributor.author | Llano, Manuel | es_ES |
dc.contributor.author | Rodríguez-Fernández, José Luis | es_ES |
dc.contributor.author | Tejedor, Reyes | es_ES |
dc.contributor.author | Avraham, Shalom | es_ES |
dc.contributor.author | Cabañas, Carlos | es_ES |
dc.contributor.author | López-Botet, Miguel | es_ES |
dc.contributor.author | Sánchez-Madrid, Francisco | es_ES |
dc.date.accessioned | 2016-08-09T09:35:07Z | - |
dc.date.available | 2016-08-09T09:35:07Z | - |
dc.date.issued | 2000-06-12 | - |
dc.identifier.citation | J Cell Biol. 149(6):1249-62 (2000) | es_ES |
dc.identifier.issn | 0021-9525 | - |
dc.identifier.uri | http://hdl.handle.net/10261/135416 | - |
dc.description | 13 p.-8 fig.-1 tab. | es_ES |
dc.description.abstract | The compartmentalization of plasma membrane proteins has a key role in regulation of lymphocyte activation and development of immunity. We found that the proline-rich tyrosine kinase-2 (PYK-2/RAFTK) colocalized with the microtubule-organizing center (MTOC) at the trailing edge of migrating natural killer (NK) cells. When polyclonal NK cells bound to K562 targets, PYK-2 translocated to the area of NK-target cell interaction. The specificity of this process was assessed with NK cell clones bearing activatory or inhibitory forms of CD94/NKG2. The translocation of PYK-2, MTOC, and paxillin to the area of NK-target cell contact was regulated upon specific recognition of target cells through NK cell receptors, controlling target cell killing. Furthermore, parallel in vitro kinase assays showed that PYK-2 was activated in response to signals that specifically triggered its translocation and NK cell mediated cytotoxicity. The overexpression of both the wt and a dominant-negative mutant of PYK-2, but not ZAP-70 wt, prevented the specific translocation of the MTOC and paxillin, and blocked the cytotoxic response of NK cells. Our data indicate that subcellular compartmentalization of PYK-2 correlates with effective signal transduction. Furthermore, they also suggest an important role for PYK-2 on the assembly of the signaling complexes that regulate the cytotoxic response. | es_ES |
dc.description.sponsorship | This work was supported by grant SAF99/0034-C01, SAF98-0006, and 2FD97-0680-C02-02 from the Ministerio de Educación y Cultura; grant 08.1/0011/97 (to F. Sánchez-Madrid), 08/005/97 (to M. López-Botet), and 08.1/0015/97 (to C. Cabañas) from the Comunidad Autónoma de Madrid;and grant SAF98/0080 (to C. Cabañas) and fellowships from the Fondo de Investigaciones Sanitarias BAE 97/5089 (to M. Nieto). J.L. Rodriguez-Fernández was supported by a “Contrato de Reincorporación” associated to grants PB94-0231, SAF98/0080, and SAF98/0006 awarded by the “Ministerio Español de Educación y Cultura”. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Rockefeller University Press | es_ES |
dc.relation.isversionof | Publisher's version | es_ES |
dc.rights | openAccess | es_ES |
dc.subject | CD94/NKG2 | es_ES |
dc.subject | Cytotoxicity | es_ES |
dc.subject | Microtubule- organizing center | es_ES |
dc.subject | Cytoskeletal proteins | es_ES |
dc.subject | HLA-E | es_ES |
dc.title | The tyrosine kinase PYK-2/RAFTK regulates natural killer (NK) cell cytotoxic response, and is translocated and activated upon specific target cell recognition and killing. | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1083/jcb.149.6.1249 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/ 10.1083/jcb.149.6.1249 | es_ES |
dc.identifier.e-issn | 1540-8140 | - |
dc.contributor.funder | Ministerio de Educación y Cultura (España) | es_ES |
dc.contributor.funder | Comunidad de Madrid | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/100012818 | es_ES |
dc.identifier.pmid | 10851022 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
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J Cell Biol-2000-Sancho-1249-62.pdf | Artículo principal | 501,17 kB | Adobe PDF | Visualizar/Abrir |
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