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Title

Detection of chromothripsis-like patterns with a custom array platform for chronic lymphocytic leukemia

AuthorsSalaverria, Itziar; Queirós, Ana C.; Colomer, Dolors; González, Marcos ; Campo, Elías; Beà, Silvia
Issue Date2015
PublisherWiley-Liss
CitationGenes Chromosomes and Cancer 54(11): 668-680 (2015)
AbstractChronic lymphocytic leukemia (CLL) is a common disease with highly variable clinical course. Several recurrent chromosomal alterations are associated with prognosis and may guide risk-adapted therapy. We have developed a targeted genome-wide array to provide a robust tool for ascertaining abnormalities in CLL and to overcome limitations of the 4-marker fluorescence in situ hybridization (FISH). DNA from 180 CLL patients were hybridized to the qChip®Hemo array with a high density of probes covering commonly altered loci in CLL (11q22-q23, 13q14, and 17p13), nine focal regions (2p15-p16.1, 2p24.3, 2q13, 2q36.3-q37.1, 3p21.31, 8q24.21, 9p21.3, 10q24.32, and 18q21.32-q21.33) and two larger regions (6q14.1-q22.31 and 7q31.33-q33). Overall, 86% of the cases presented copy number alterations (CNA) by array. There was a high concordance of array findings with FISH (84% sensitivity, 100% specificity); all discrepancies corresponded to subclonal alterations detected only by FISH. A chromothripsis-like pattern was detected in eight cases. Three showed concomitant shattered 5p with gain of TERT along with isochromosome 17q. Presence of 11q loss was associated with shorter time to first treatment (P=0.003), whereas 17p loss, increased genomic complexity, and chromothripsis were associated with shorter overall survival (P<0.001, P=0.001, and P=0.02, respectively). In conclusion, we have validated a targeted array for the diagnosis of CLL that accurately detects, in a single experiment, all relevant CNAs, genomic complexity, chromothripsis, copy number neutral loss of heterozygosity, and CNAs not covered by the FISH panel. This test may be used as a practical tool to stratify CLL patients for routine diagnostics or clinical trials.
DescriptionThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial License.-- et al.
Publisher version (URL)http://dx.doi.org/10.1002/gcc.22277
URIhttp://hdl.handle.net/10261/134803
DOI10.1002/gcc.22277
Identifiersdoi: 10.1002/gcc.22277
e-issn: 1098-2264
issn: 1045-2257
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