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The activation of the Sox2 RR2 pluripotency transcriptional reporter in human breast cancer cell lines is dynamic and labels cells with higher tumorigenic potential

AuthorsIglesias, Juan Manuel; Hernández-García, Susana; Pandiella, Atanasio ; García Martín, Ángel
Breast cancer stem cell
Issue Date2014
PublisherFrontiers Media
CitationFrontiers in Oncology 4: 308 (2014)
AbstractThe striking similarity displayed at the mechanistic level between tumorigenesis and the generation of induced pluripotent stem cells and the fact that genes and pathways relevant for embryonic development are reactivated during tumor progression highlights the link between pluripotency and cancer. Based on these observations, we tested whether it is possible to use a pluripotency-associated transcriptional reporter, whose activation is driven by the SRR2 enhancer from the Sox2 gene promoter (named S4+ reporter), to isolate cancer stem cells (CSCs) from breast cancer cell lines. The S4+ pluripotency transcriptional reporter allows the isolation of cells with enhanced tumorigenic potential and its activation was switched on and offin the cell lines studied, reflecting a plastic cellular process. Microarray analysis comparing the populations in which the reporter construct is active versus inactive showed that positive cells expressed higher mRNA levels of cytokines (IL-8, IL-6, TNF) and genes (such as ATF3, SNAI2, and KLF6) previously related with the CSC phenotype in breast cancer.
DescriptionThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).-- et al.
Publisher version (URL)http://dx.doi.org/10.3389/fonc.2014.00308
Identifiersdoi: 10.3389/fonc.2014.00308
e-issn: 2234-943X
Appears in Collections:(IBMCC) Artículos
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