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Development of microsatellite markers in the deep-dea cup coral Desmophyllum dianthus by 454 sequencing and cross-species amplifications in Scleractinia Order

AuthorsAddamo, Anna Maria ; García-Jiménez, Ricardo; Taviani, Marco; Machordom, Annie
Deep sea coral
High-throughput sequencing
Population structure
Issue DateMay-2015
PublisherOxford University Press
CitationJournal of Heredity 106(3): 322-330 (2015)
AbstractMicrosatellite loci were isolated for the first time for the deep-sea coral Desmophyllum dianthus, using 454 GS-FLX Titanium pyrosequencing. We developed conditions for amplifying 24 markers in 10 multiplex reactions. Three to 16 alleles per locus were detected across 25 samples analyzed from Santa Maria di Leuca coral province (Mediterranean Sea). For the 24 polymorphic loci, observed and expected heterozygosities ranged from 0.211 to 0.880 and 0.383 to 0.910, respectively; 3 loci deviated from Hardy-Weinberg equilibrium, after null allele and sequential Holm-Bonferroni corrections. These newly isolated microsatellites are very useful genetic markers that provide data for future conservation strategies. Cross-amplification of these microsatellites, tested in 46 coral species, representing 40 genera, and 10 families of the phylum Cnidaria, produced informative allelic profiles for 1 to 24 loci. The utility of extending analyses to cross-species amplifications is also discussed.
Description[EN] This adaptation text reproduces chapter III of the dissertation “Results”: Systematics and phylogeography of the deep-sea coral Desmophyllum dianthus (Anthozoa, Hexacorallia): Morphological and molecular evidences, de Anna Maria Addamo (2014), http://hdl.handle.net/10261/134194
[ES] Este artículo es una adaptación del capítulo III de los “Resultados” de la tesis doctoral: Sistemática y filogeografía del coral de profundidad Desmophyllum dianthus (Anthozoa, Hexacorallia): Indicios morfológicos y moleculares, de Anna Maria Addamo (2014), http://hdl.handle.net/10261/134194
Publisher version (URL)http://dx.doi.org/10.1093/jhered/esv010
Appears in Collections:(MNCN) Artículos
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