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Title

CD20 positive cells are undetectable in the majority of multiple myeloma cell lines and are not associated with a cancer stem cell phenotype

AuthorsPaíno, Teresa ; Ocio, Enrique M. ; Paiva, Bruno; San-Segundo, Laura; Garayoa, Mercedes ; Gutiérrez, Norma Carmen; Sarasquete, María Eugenia; Pandiella, Atanasio ; Orfao, Alberto ; San Miguel, Jesús F.
Issue Date2012
PublisherFerrata Storti Foundation
CitationHaematologica 97(7): 1110-1114 (2012)
AbstractAlthough new therapies have doubled the survival of multiple myeloma patients, this remains an incurable disease. It has been postulated that the so-called myeloma cancer stem cells would be responsible for tumor initiation and relapse but their unequivocal identification remains unclear. Here, we investigated in a panel of myeloma cell lines the presence of CD20(+) cells harboring a stem-cell phenotype. Thus, only a small population of CD20(dim+) cells (0.3%) in the RPMI-8226 cell line was found. CD20(dim+) RPMI-8226 cells expressed the plasma cell markers CD38 and CD138 and were CD19(-)CD27(-). Additionally, CD20(dim+) RPMI-8226 cells did not exhibit stem-cell markers as shown by gene expression profiling and the aldehyde dehydrogenase assay. Furthermore, we demonstrated that CD20(dim+) RPMI-8226 cells are not essential for CB17-SCID mice engraftment and show lower self-renewal potential than the CD20(-) RPMI-8226 cells. These results do not support CD20 expression for the identification of myeloma cancer stem cells.
DescriptionThis is an open-access paper.
Publisher version (URL)http://dx.doi.org/10.3324/haematol.2011.057372
URIhttp://hdl.handle.net/10261/134103
DOI10.3324/haematol.2011.057372
Identifiersdoi: 10.3324/haematol.2011.057372
issn: 0390-6078
e-issn: 1592-8721
Appears in Collections:(IBMCC) Artículos
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