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Título: | Amplified and Homozygously Deleted Genes in Glioblastoma: Impact on Gene Expression Levels |
Autor: | Crespo, Inês; Nieto, Ana B.; Domingues, Patricia H.; Vital, Ana Luísa; Orfao, Alberto CSIC ORCID ; Tabernero, María D. | Fecha de publicación: | 2012 | Editor: | Public Library of Science | Citación: | PLoS ONE 7(9): e46088 (2012) | Resumen: | [Background]: Glioblastoma multiforme (GBM) displays multiple amplicons and homozygous deletions that involve relevant pathogenic genes and other genes whose role remains unknown. [Methodology]: Single-nucleotide polymorphism (SNP)-arrays were used to determine the frequency of recurrent amplicons and homozygous deletions in GBM (n = 46), and to evaluate the impact of copy number alterations (CNA) on mRNA levels of the genes involved. [Principal Findings]: Recurrent amplicons were detected for chromosomes 7 (50%), 12 (22%), 1 (11%), 4 (9%), 11 (4%), and 17 (4%), whereas homozygous deletions involved chromosomes 9p21 (52%) and 10q (22%). Most genes that displayed a high correlation between DNA CNA and mRNA levels were coded in the amplified chromosomes. For some amplicons the impact of DNA CNA on mRNA expression was restricted to a single gene (e.g., EGFR at 7p11.2), while for others it involved multiple genes (e.g., 11 and 5 genes at 12q14.1-q15 and 4q12, respectively). Despite homozygous del(9p21) and del(10q23.31) included multiple genes, association between these DNA CNA and RNA expression was restricted to the MTAP gene. [Conclusions]: Overall, our results showed a high frequency of amplicons and homozygous deletions in GBM with variable impact on the expression of the genes involved, and they contributed to the identification of other potentially relevant genes. | Descripción: | This is an open-access article distributed under the terms of the Creative Commons Attribution License.-- et al. | Versión del editor: | http://dx.doi.org/10.1371/journal.pone.0046088 | URI: | http://hdl.handle.net/10261/134085 | DOI: | 10.1371/journal.pone.0046088 | Identificadores: | doi: 10.1371/journal.pone.0046088 issn: 1932-6203 |
Aparece en las colecciones: | (IBMCC) Artículos |
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Gene Expression Levels.pdf | 1,08 MB | Adobe PDF | Visualizar/Abrir |
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