Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/131778
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

A second chromosomal copy of the catA gene endows Pseudomonas putida mt-2 with an enzymatic safety valve for excess of catechol

AutorJiménez, Jose I.; Pérez-Pantoja, Danilo; Chavarría, Max; Díaz, Eduardo CSIC ORCID ; Lorenzo, Víctor de CSIC ORCID
Palabras claveCatechols
Biodegradation
Pseudomonas
TOL plasmid
Toxicity
Fecha de publicaciónjun-2014
EditorBlackwell Publishing
CitaciónEnvironmental Microbiology, 16(6) : 1767-1778 (2014)
ResumenPseudomonas putida mt-2 harbors two different routes for catabolism of catechol, namely one meta pathway encoded by the xyl genes of the TOL plasmid pWW0 and one ortho pathway determined by the chromosomal ben and cat genes. P. putida mt-2 has a second chromosomal copy of the catA gene (named catA2) located downstream of the ben operon that encodes an additional catechol-1,2-dioxygenase. The metabolic and regulatory phenotypes of strains lacking one enzyme, the other and both of them in cells with and without the TOL plasmid were evaluated. The data consistently indicated that induction of the ortho pathway by benzoate plasmid-less strain P. putida KT2440 led to catechol surplus, the toxicity of which at high concentrations being counteracted by CatA2. Cells carrying pWW0 but lacking catA2 experienced both a rapid loss of the plasmid when grown on benzoate (a substrate of the lower pathway) and a slowdown of their growth rate when cultured with benzylalcohol (a substrate converted to benzoate by the upper pathway). These data reveal the role of CatA2 as a type of metabolic safety valve for excess catechol that alleviates the metabolic conflict generated by simultaneous expression of the meta and ortho pathways, thereby facilitating their co-existence.
Versión del editorhttp://dx.doi.org/10.1111/1462-2920.12361
URIhttp://hdl.handle.net/10261/131778
DOI10.1111/1462-2920.12361
ISSN1462-2912
E-ISSN1462-2920
Aparece en las colecciones: (CNB) Artículos

Ficheros en este ítem:
Fichero Descripción Tamaño Formato
8.291_A Second Chromosomal.pdfArtículo principal9,03 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo

CORE Recommender

SCOPUSTM   
Citations

28
checked on 16-mar-2024

WEB OF SCIENCETM
Citations

29
checked on 24-feb-2024

Page view(s)

351
checked on 28-mar-2024

Download(s)

388
checked on 28-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.