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Phosphatidylcholine:diacylglycerol cholinephosphotransferase gene from Sunflower (Helianthus annuus L.)

AuthorsMapelli-Brahm, A.; Salas, Joaquín J. ; Martínez-Force, Enrique ; Garcés Mancheño, Rafael ; Venegas-Calerón, Mónica
Issue Date5-Jul-2015
AbstractPlant oils have a wide number of applications including food, feed, biofuel and industrial uses. The fatty acids composition and their distribution within the triacylglycerol (TAG) molecules determine the physical, chemical and nutritional properties of plants oils. Phospatidylcholine (PC) plays an important role in TAG synthesis. Fatty acids esterified in the position sn-2 of PC can suffer different modifications like desaturation, hydroxylation or epoxidation. In this regard, phosphatidylcholine:diacylglycerol cholinephosphotransferase (PDCT) catalyzes the reversible transfer of the phosphocholine headgroup from PC to diacylglycerol (DAG). Thus, through PDCT reactions, the acyl groups on DAG enter on PC and then return to DAG after their modifications on PC. Because TAG can be assembly with PC-derived DAG, PDCT could play an important role in the synthesis of TAG with modified fatty acids in its sn-2 position. In this work the gene HaROD encoding for a AtPDCT protein homologous has been cloned and sequenced from sunflower developing seeds. Informatics analysis of its sequence and further details of functional characterization of this gene are reported. Results in this research could contribute to a better understanding of the synthesis of TAGs in oil crops, and to find novel paths for the production of oils with new compositions and applications.
Appears in Collections:(IG) Comunicaciones congresos
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