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dc.contributor.authorSoler, María-
dc.contributor.authorEstévez, M. Carmen-
dc.contributor.authorÁlvarez, Mar-
dc.contributor.authorOtte, Marinus A.-
dc.contributor.authorSepúlveda, Borja-
dc.contributor.authorLechuga, Laura M.-
dc.identifierdoi: 10.3390/s140202239-
dc.identifierissn: 1424-8220-
dc.identifier.citationSensors 14(2): 2239-2258 (2014)-
dc.descriptionThis is an open access article distributed under the Creative Commons Attribution License (CC BY).-
dc.descriptionThis article belongs to the Special Issue Plasmonics and Nanoplasmonics Biosensors.-
dc.description.abstractDesign of an optimal surface biofunctionalization still remains an important challenge for the application of biosensors in clinical practice and therapeutic follow-up. Optical biosensors offer real-time monitoring and highly sensitive label-free analysis, along with great potential to be transferred to portable devices. When applied in direct immunoassays, their analytical features depend strongly on the antibody immobilization strategy. A strategy for correct immobilization of antibodies based on the use of ProLinker™ has been evaluated and optimized in terms of sensitivity, selectivity, stability and reproducibility. Special effort has been focused on avoiding antibody manipulation, preventing nonspecific adsorption and obtaining a robust biosurface with regeneration capabilities. ProLinker™-based approach has demonstrated to fulfill those crucial requirements and, in combination with PEG-derivative compounds, has shown encouraging results for direct detection in biological fluids, such as pure urine or diluted serum. Furthermore, we have implemented the ProLinker™ strategy to a novel nanoplasmonic-based biosensor resulting in promising advantages for its application in clinical and biomedical diagnosis.-
dc.description.sponsorshipThe work has been done thanks to the MULTIBIOPLAS project (TEC2099-08729) and the INNBIOD project (INNPACTO Subprogram, OPT-2011-1429-010000) from Spanish Ministry of Economy and Competitiveness. M. S. acknowledges financial support from “Programa de Formación de Personal Investigador (FPI)” from the Spanish Ministry of Economy and Competitiveness.-
dc.description.sponsorshipWe acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI).-
dc.publisherMultidisciplinary Digital Publishing Institute-
dc.relation.isversionofPublisher's version-
dc.subjectClinical diagnosis-
dc.subjectGold nanodisks-
dc.subjectNanoplasmonic biosensor-
dc.subjectSPR biosensor-
dc.subjectDirect immunoassay-
dc.subjectAntibody immobilization-
dc.titleDirect detection of protein biomarkers in human fluids using site-specific antibody immobilization strategies-
dc.description.versionPeer Reviewed-
dc.contributor.funderConsejo Superior de Investigaciones Científicas (España)-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
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