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Direct detection of protein biomarkers in human fluids using site-specific antibody immobilization strategies

AuthorsSoler, María ; Estévez, M. Carmen ; Álvarez, Mar ; Otte, Marinus A. ; Sepúlveda, Borja ; Lechuga, Laura M.
KeywordsClinical diagnosis
Gold nanodisks
Nanoplasmonic biosensor
SPR biosensor
Direct immunoassay
Antibody immobilization
Issue Date2014
PublisherMultidisciplinary Digital Publishing Institute
CitationSensors 14(2): 2239-2258 (2014)
AbstractDesign of an optimal surface biofunctionalization still remains an important challenge for the application of biosensors in clinical practice and therapeutic follow-up. Optical biosensors offer real-time monitoring and highly sensitive label-free analysis, along with great potential to be transferred to portable devices. When applied in direct immunoassays, their analytical features depend strongly on the antibody immobilization strategy. A strategy for correct immobilization of antibodies based on the use of ProLinker™ has been evaluated and optimized in terms of sensitivity, selectivity, stability and reproducibility. Special effort has been focused on avoiding antibody manipulation, preventing nonspecific adsorption and obtaining a robust biosurface with regeneration capabilities. ProLinker™-based approach has demonstrated to fulfill those crucial requirements and, in combination with PEG-derivative compounds, has shown encouraging results for direct detection in biological fluids, such as pure urine or diluted serum. Furthermore, we have implemented the ProLinker™ strategy to a novel nanoplasmonic-based biosensor resulting in promising advantages for its application in clinical and biomedical diagnosis.
DescriptionThis is an open access article distributed under the Creative Commons Attribution License (CC BY).
This article belongs to the Special Issue Plasmonics and Nanoplasmonics Biosensors.
Publisher version (URL)http://dx.doi.org/10.3390/s140202239
Identifiersdoi: 10.3390/s140202239
issn: 1424-8220
Appears in Collections:(CIN2) Artículos
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