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Direct detection of protein biomarkers in human fluids using site-specific antibody immobilization strategies
|Authors:||Soler, María ; Estévez, M. Carmen ; Álvarez, Mar ; Otte, Marinus A. ; Sepúlveda, Borja ; Lechuga, Laura M.|
|Publisher:||Multidisciplinary Digital Publishing Institute|
|Citation:||Sensors 14(2): 2239-2258 (2014)|
|Abstract:||Design of an optimal surface biofunctionalization still remains an important challenge for the application of biosensors in clinical practice and therapeutic follow-up. Optical biosensors offer real-time monitoring and highly sensitive label-free analysis, along with great potential to be transferred to portable devices. When applied in direct immunoassays, their analytical features depend strongly on the antibody immobilization strategy. A strategy for correct immobilization of antibodies based on the use of ProLinker™ has been evaluated and optimized in terms of sensitivity, selectivity, stability and reproducibility. Special effort has been focused on avoiding antibody manipulation, preventing nonspecific adsorption and obtaining a robust biosurface with regeneration capabilities. ProLinker™-based approach has demonstrated to fulfill those crucial requirements and, in combination with PEG-derivative compounds, has shown encouraging results for direct detection in biological fluids, such as pure urine or diluted serum. Furthermore, we have implemented the ProLinker™ strategy to a novel nanoplasmonic-based biosensor resulting in promising advantages for its application in clinical and biomedical diagnosis.|
|Description:||This is an open access article distributed under the Creative Commons Attribution License (CC BY).|
This article belongs to the Special Issue Plasmonics and Nanoplasmonics Biosensors.
|Publisher version (URL):||http://dx.doi.org/10.3390/s140202239|
|Appears in Collections:||(CIN2) Artículos|
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