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Título: | Direct detection of protein biomarkers in human fluids using site-specific antibody immobilization strategies |
Autor: | Soler, María CSIC ORCID; Estévez, M. Carmen CSIC ORCID ; Álvarez, Mar CSIC ORCID; Otte, Marinus A. CSIC; Sepúlveda, Borja CSIC ORCID; Lechuga, Laura M. CSIC ORCID | Palabras clave: | Clinical diagnosis Gold nanodisks Nanoplasmonic biosensor SPR biosensor Direct immunoassay ProLinker™ Antibody immobilization |
Fecha de publicación: | 2014 | Editor: | Multidisciplinary Digital Publishing Institute | Citación: | Sensors 14(2): 2239-2258 (2014) | Resumen: | Design of an optimal surface biofunctionalization still remains an important challenge for the application of biosensors in clinical practice and therapeutic follow-up. Optical biosensors offer real-time monitoring and highly sensitive label-free analysis, along with great potential to be transferred to portable devices. When applied in direct immunoassays, their analytical features depend strongly on the antibody immobilization strategy. A strategy for correct immobilization of antibodies based on the use of ProLinker™ has been evaluated and optimized in terms of sensitivity, selectivity, stability and reproducibility. Special effort has been focused on avoiding antibody manipulation, preventing nonspecific adsorption and obtaining a robust biosurface with regeneration capabilities. ProLinker™-based approach has demonstrated to fulfill those crucial requirements and, in combination with PEG-derivative compounds, has shown encouraging results for direct detection in biological fluids, such as pure urine or diluted serum. Furthermore, we have implemented the ProLinker™ strategy to a novel nanoplasmonic-based biosensor resulting in promising advantages for its application in clinical and biomedical diagnosis. | Descripción: | This is an open access article distributed under the Creative Commons Attribution License (CC BY). This article belongs to the Special Issue Plasmonics and Nanoplasmonics Biosensors. |
Versión del editor: | http://dx.doi.org/10.3390/s140202239 | URI: | http://hdl.handle.net/10261/131510 | DOI: | 10.3390/s140202239 | Identificadores: | doi: 10.3390/s140202239 issn: 1424-8220 |
Aparece en las colecciones: | (CIN2) Artículos |
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immobilizationstrategies.pdf | 864,54 kB | Adobe PDF | Visualizar/Abrir |
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