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Molecular Characterization of Resistance-Nodulation-Division Transporters from Solvent- and Drug-Resistant Bacteria in Petroleum-Contaminated Soil

AuthorsMeguro, Norika; Kodama, Yumiko; Gallegos, María Trinidad ; Watanabe, Kazuya
KeywordsMolecular mechanisms
Contaminated soil
Organic solvents
Solvent-resistant bacteria
Issue DateJan-2005
PublisherAmerican Society for Microbiology
CitationApplied and Environmental Microbiology 71 (1): 580–586 (2005)
AbstractPCR assays for analyzing resistance-nodulation-division transporters from solvent- and drug-resistant bacteria in soil were developed. Sequence analysis of amplicons showed that the PCR successfully retrieved transporter gene fragments from soil. Most of the genes retrieved from petroleum-contaminated soils formed a cluster (cluster PCS) that was distantly related to known transporter genes. Competitive PCR showed that the abundance of PCS genes is increased in petroleum-contaminated soil.
Publisher version (URL)http://dx.doi.org/10.1128/AEM.71.1.580–586.2005
Appears in Collections:(EEZ) Artículos
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