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dc.contributor.authorAlcolea, Pedro J.es_ES
dc.contributor.authorAlonso, Anaes_ES
dc.contributor.authorMoreno-Izquierdo, Miguel A.es_ES
dc.contributor.authorDegayón, M. A.es_ES
dc.contributor.authorMoreno-Córdoba, Inmaculadaes_ES
dc.contributor.authorLarraga, Vicentees_ES
dc.identifier.citationPLoS One. 11(3):e0150172 (2016)es_ES
dc.description17 p.-6 fig.-1 tab.es_ES
dc.description.abstractLeishmania infantum is one of the species responsible for visceral leishmaniasis. This species is distributed basically in the Mediterranean basin. A recent outbreak in humans has been reported in Spain. Axenic cultures are performed for most procedures with Leishmania spp. promastigotes. This model is stable and reproducible and mimics the conditions of the gut of the sand fly host, which is the natural environment of promastigote development. Culture media are undefined because they contain mammalian serum, which is a rich source of complex lipids and proteins. Serum deprivation slows down the growth kinetics and therefore, yield in biomass. In fact, we have confirmed that the growth rate decreases, as well as infectivity. Ploidy is also affected. Regarding the transcriptome, a high-throughput approach has revealed a low differential expression rate but important differentially regulated genes. The most remarkable profiles are: up-regulation of the GINS Psf3, the fatty acyl-CoA synthase (FAS1), the glyoxylase I (GLO1), the hydrophilic surface protein B (HASPB), the methylmalonyl-CoA epimerase (MMCE) and an amastin gene; and down-regulation of the gPEPCK and the arginase. Implications for metabolic adaptations, differentiation and infectivity are discussed herein.es_ES
dc.description.sponsorshipPA thanks CSIC for the I3P-BPD2003-1 grant and two contracts of employment at a position included in the A1 group (respectively developed from January 16th to July 23rd 2008 and from October 16th 2008 to April 15th 2009). MAD thanks the Spanish Ministry of Economy and Competitiveness for the FPI Pre-doctoral fellowship BES-2011-047361.es_ES
dc.publisherPublic Library of Sciencees_ES
dc.relation.isversionofPublisher's versiones_ES
dc.titleSerum removal from culture induces growth arrest, ploidy alteration, decrease in infectivity and differential expression of crucial genes in Leishmania infantum promastigoteses_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/ 10.1371/journal.pone.0150172es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
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