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dc.contributor.authorMartín, Susana-
dc.contributor.authorSambade, Adrián-
dc.contributor.authorRubio, Luis-
dc.contributor.authorVives, María C.-
dc.contributor.authorMoya, Patricia-
dc.contributor.authorGuerri, José-
dc.contributor.authorElena, Santiago F.-
dc.contributor.authorMoreno, Pedro-
dc.identifier.citationJournal of General Virology 90: 1527-1538 (2009)en_US
dc.description30 páginas, 3 figuras, 2 tablas.-
dc.description.abstractThe genetic variation of Citrus tristeza virus (CTV) was analyzed comparing the predominant sequence variants in seven genomic regions (p33, p65, p61, p18, p13, p20, and p23) of 18 pathogenically distinct isolates from seven different countries. Analyses of the selective constraints acting on each codon suggest that most regions were under purifying selection. Phylogenetic analysis show diverse patterns of molecular evolution for different genomic regions. A first clade composed by isolates genetically close to the reference mild isolates T385 or T30 was inferred from all genomic regions. A second clade, mostly comprising virulent isolates, was defined from regions p33, p65, p13, p20, and p23. For regions p65, p61, p18, p13, and p23 a third clade that mostly included South American isolates could not be related with any reference genotype. Phylogenetic relationships among isolates did not reflect their geographical origin, suggesting significant gene flow between geographically distant areas. Incongruent phylogenetic trees for different genomic regions suggested recombination events, an extreme that was supported by several recombination-detecting methods. A phylogenetic network incorporating the effect of recombination showed an explosive radiation pattern for the evolution of some isolates and grouped isolates by virulence. Taken together, the above results suggest that negative selection, gene flow, sequence recombination, and virulence may be important factors driving CTV evolution.en_US
dc.description.sponsorshipS. Martín and A. Sambade were recipient of fellowships from the Spanish Ministerio de Ciencia e Innovación and Generalitat Valenciana, respectively. This work was supported in part by grants AGL2004-05099/AGR and AGL2007-61885/AGR (work at IVIA) and BFU2006-14819-C02-01/BMC (work at IBMCP) from the Ministerio de Ciencia e Innovación.en_US
dc.format.extent472319 bytes-
dc.publisherSociety for General Microbiologyen_US
dc.subjectVirus evolutionen_US
dc.subjectMolecular evolutionen_US
dc.subjectGenetic variabilityen_US
dc.subjectMaximum likelihooden_US
dc.subjectMolecular epidemiologyen_US
dc.subjectSelective constraintsen_US
dc.titleContribution of recombination and selection to molecular evolution of Citrus tristeza virusen_US
dc.description.peerreviewedPeer revieweden_US
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