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Título: | Evaluation of styrene-divinylbenzene beads as a support to immobilize lipases |
Autor: | García-Galán, Cristina; Barbosa, Oveimar CSIC ORCID; Hernández Sánchez, Karel CSIC ORCID; Dos Santos, José C. S.; Rodrigues, Rafael C.; Fernández-Lafuente, Roberto CSIC ORCID | Palabras clave: | lipase immobilization, modulation of lipase activity, interfacial activation, styrene divinylbencene matrix | Fecha de publicación: | 10-jun-2014 | Editor: | Molecular Diversity Preservation International | Citación: | Molecules 19: 7629-7645 (2014) | Resumen: | A commercial and very hydrophobic styrene-divinylbenzene matrix, MCI GEL® CHP20P, has been compared to octyl-Sepharose® beads as support to immobilize three different enzymes: lipases from Thermomyces lanuginosus (TLL) and from Rhizomucor miehie (RML) and Lecitase ® Ultra, a commercial artificial phospholipase. The immobilization mechanism on both supports was similar: interfacial activation of the enzymes versus the hydrophobic surface of the supports. Immobilization rate and loading capacity is much higher using MCI GEL® CHP20P compared to octyl-Sepharose® (87.2 mg protein/g of support using TLL, 310 mg/g using RML and 180 mg/g using Lecitase® Ultra). The thermal stability of all new preparations is much lower than that of the standard octyl-Sepharose® immobilized preparations, while the opposite occurs when the inactivations were performed in the presence of organic co-solvents. Regarding the hydrolytic activities, the results were strongly dependent on the substrate and pH of measurement. Octyl-Sepharose ® immobilized enzymes were more active versus p-NPB than the enzymes immobilized on MCI GEL® CHP20P, while RML became 700-fold less active versus methyl phenylacetate. Thus, the immobilization of a lipase on this matrix needs to be empirically evaluated, since it may present very positive effects in some cases while in other cases it may have very negative ones. © 2014 by the authors. | Versión del editor: | http://dx.doi.org/10.3390/molecules19067629 | URI: | http://hdl.handle.net/10261/130945 | DOI: | 10.3390/molecules19067629 | Identificadores: | issn: 1420-3049 |
Aparece en las colecciones: | (ICP) Artículos (IQAC) Artículos |
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Styrene-Divinylbenzene_Beads_Garcia-Galan.pdf | 240 kB | Adobe PDF | Visualizar/Abrir |
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