Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/130945
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

Evaluation of styrene-divinylbenzene beads as a support to immobilize lipases

AutorGarcía-Galán, Cristina; Barbosa, Oveimar CSIC ORCID; Hernández Sánchez, Karel CSIC ORCID; Dos Santos, José C. S.; Rodrigues, Rafael C.; Fernández-Lafuente, Roberto CSIC ORCID
Palabras clavelipase immobilization, modulation of lipase activity, interfacial activation, styrene divinylbencene matrix
Fecha de publicación10-jun-2014
EditorMolecular Diversity Preservation International
CitaciónMolecules 19: 7629-7645 (2014)
ResumenA commercial and very hydrophobic styrene-divinylbenzene matrix, MCI GEL® CHP20P, has been compared to octyl-Sepharose® beads as support to immobilize three different enzymes: lipases from Thermomyces lanuginosus (TLL) and from Rhizomucor miehie (RML) and Lecitase ® Ultra, a commercial artificial phospholipase. The immobilization mechanism on both supports was similar: interfacial activation of the enzymes versus the hydrophobic surface of the supports. Immobilization rate and loading capacity is much higher using MCI GEL® CHP20P compared to octyl-Sepharose® (87.2 mg protein/g of support using TLL, 310 mg/g using RML and 180 mg/g using Lecitase® Ultra). The thermal stability of all new preparations is much lower than that of the standard octyl-Sepharose® immobilized preparations, while the opposite occurs when the inactivations were performed in the presence of organic co-solvents. Regarding the hydrolytic activities, the results were strongly dependent on the substrate and pH of measurement. Octyl-Sepharose ® immobilized enzymes were more active versus p-NPB than the enzymes immobilized on MCI GEL® CHP20P, while RML became 700-fold less active versus methyl phenylacetate. Thus, the immobilization of a lipase on this matrix needs to be empirically evaluated, since it may present very positive effects in some cases while in other cases it may have very negative ones. © 2014 by the authors.
Versión del editorhttp://dx.doi.org/10.3390/molecules19067629
URIhttp://hdl.handle.net/10261/130945
DOI10.3390/molecules19067629
Identificadoresissn: 1420-3049
Aparece en las colecciones: (ICP) Artículos
(IQAC) Artículos




Ficheros en este ítem:
Fichero Descripción Tamaño Formato
Styrene-Divinylbenzene_Beads_Garcia-Galan.pdf240 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo

CORE Recommender

PubMed Central
Citations

11
checked on 27-mar-2024

SCOPUSTM   
Citations

62
checked on 23-mar-2024

WEB OF SCIENCETM
Citations

62
checked on 29-feb-2024

Page view(s)

248
checked on 28-mar-2024

Download(s)

193
checked on 28-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


Artículos relacionados:


Este item está licenciado bajo una Licencia Creative Commons Creative Commons