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Título

Organotypic Brain cultures as a model for neurobrucellosis research

AutorSangari, Félix J. CSIC ORCID; Remuzgo-Martínez, Sara; Ramos-Vivas, José; Valdizán, Elsa M. CSIC ORCID; Romero-Presno, Beatriz CSIC; Seoane, Asunción CSIC; García-Lobo, Juan M. CSIC ORCID
Palabras claveBrucellosis
Fecha de publicación2014
CitaciónBrucellosis 2014
ResumenBrucellosis is a zoonotic disease caused in humans by infection with diferent species of the genus Brucella spp. The most characteristic symptoms are an undulating fever, sweating, and joint and muscle pains, the classic brucellosis triad. One of the life-threatening complications of brucellosis is neurobrucellosis, that has been identified in up to one-third of pacients with cofirmed Brucella infection. The study of the mechanisms of infectious diseases in the brain has classically relied on the use of animal models and two-dimensional (2D) cell cultures. However, 2D monolayers lack of the structural complexity and the physiological relevance of the in vivo tissue preparations, since they are limited predictors of the infection process. In contrast, the use of animal models is time-consuming and depends on the availability of costly animal facilities. The introduction of the ex-vivo nervous system model (3D organotypic slice cultures) has incorporated a fundamental experimental tool in this field of study. Key features of the ex vivo model include well-defined cellular architecture, the presence of axonal projections, and the preservation of the in vivo 3D organization and long-term thickness of the preparation. Thus, the model mimics the morphological and functional features of the in vivo parental tissues. Brain slice cultures have been used to study infections of the CNS with several pathogens, and we have tested their ability to support Brucella infection and replicate some of the in vivo findings. Organotypic explants were obtained from newborn (7-10-day old) Sprague Dawley rats, and infected with B. abortus 2308, and several avirulent mutant strains. The kinetics of the bacterial infection was determined, as well as expression of several markers associated with in vivo infection.
DescripciónResumen del póster presentado al Brucellosis 2014 International Research Conference, celebrado en Berlín (Alemania) del 9 al 12 de septiembre de 2014.
URIhttp://hdl.handle.net/10261/130760
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