English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/130675
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Inhibition of Type IV secretion ATPase TrwD by unsaturated fatty acids as a potential tool to prevent wide spread dissemination of antibiotic resistance genes

AuthorsGarcía-Cazorla, Yolanda; Ripoll-Rozada, Jorge ; Machón, Cristina ; Getino, María; Cruz, Fernando de la ; Cabezón, Elena ; Arechaga, Ignacio
Issue Date2014
PublisherSociedad Española de Bioquímica y Biología Molecular
CitationXXXVII Congreso de la SEBBM (2014)
AbstractAntibiotic resistance has become a major health issue. Unfortunately, the development of new antibiotics is being very slow and expensive and we are running out of weapons to fi ght against the appearance of multi-resistant bugs. Bacterial conjugation is the main mechanism for the wide spread dissemination of antibiotic resistance genes and, therefore, the search of specific inhibitors of conjugation (COINs) could become a brand new strategy in this warfare. In the search of potential COINs, previous studies have reported that unsaturated fatty acids (uFAs) were able to inhibit conjugation. Based on these studies we have looked for the molecular targets of these compounds and we have found that the Type IV secretion ATPase TrwD is inhibited by linoleic acid and 2-alkynoic fatty acids, such as the 2-hexadecynoic acid. These two uFAs compounds were the most effective inhibitors in R388 plasmid conjugation. The inhibitory effect is specific for the traffic ATPase TrwD, as the remaining ATPases of the Type IV secretion system are unaffected by both uFAs and 2-AFAs. We have characterized the inhibition mechanism and we have found that in both cases it is a non-competitive inhibition, as the affinities for the substrate (ATP) and product (ADP) are not altered in the presence of the fatty acids. Altogether, these results could open new avenues in the development of new strategies to prevent the dissemination of antibiotic resistance genes.
DescriptionResumen del póster presentado al XXXVII Congreso de la Sociedad Española de Bioquímica y Biología Molecular, celebrado en Granada del 9 al 12 de septiembre de 2014.
URIhttp://hdl.handle.net/10261/130675
Appears in Collections:(IBBTEC) Comunicaciones congresos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.