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Distinct serum proteome profiles associated with collagen-induced arthritis, and complete Freund's adjuvant-induced inflammation in CD38–/– mice

AuthorsRosal-Vela, Antonio; García-Rodríguez, Sonia ; Postigo, Jorge; Iglesias, Marcos ; Merino, Jesús; Merino, Ramón ; Zubiaur, Mercedes; Sancho, Jaime
Issue Date2014
PublisherSociedad Española de Bioquímica y Biología Molecular
CitationXXXVII Congreso de la SEBBM (2014)
AbstractCollagen-type-II-induced arthritis (CIA) is milder in CD38-/- than in WT mice. Their serum samples were treated with ProteoMiner-beads to equalize protein concentrations resulting in a signifi cant enrichment in proteins from extracellular vesicles (blood microparticles, exosomes). ProteoMinerequalized samples were subjected to 2D-DiGE and MS-MALDI-TOF/TOF analyses to identify proteins that were differentially expressed in CD38-/- versus WT mice either with arthritis (Col.II+/CIA+), with no arthritis (Col. II+/CIA-), or with inflmmation (CFA-treated). Altered proteins included those involved in acute phase response (SAA1), inflammation (B2MG, HABP2, Hemoglobin), complement activation (Ficolins, C4-B fragments, C1qb, C3-b-chain), polyclonal B-cell activation (Ig-kappa-light-chain) and lipid metabolism (APOE, APOAI, APOAII, APOAIV, APOJ/Clusterin). Of the proteins that changed in abundance, Hemoglobin and APOE could be indicative of the milder pathological process found in CIA+CD38-/- mice, whereas another set of proteins such as HABP2, Ig-kappa-light-chain, SAA1, Ficolin-1, and Ficolin-2 were clearly associated to the stronger response of WT mice to either collagen immunization or CFA-treatment. Multivariate analyses revealed that the differential protein abundance of 28 distinct protein spots was able to discriminate between CIA+ and CIA- mice, independently of their genetic background. This approach is suitable for the identifi cation of arthritis, or infl ammation serum proteome signatures in mice with distinct genetic backgrounds.
DescriptionResumen del póster presentado al XXXVII Congreso de la Sociedad Española de Bioquímica y Biología Molecular, celebrado en Granada del 9 al 12 de septiembre de 2014.
Appears in Collections:(IBBTEC) Comunicaciones congresos
(IPBLN) Comunicaciones congresos
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