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2D-DiGE/MS analysis allows the identification of a distinct serum protein profile in CD38-deficient mice associated with either collagen-induced arthritis or inflammation

AuthorsRosal-Vela, Antonio; García-Rodríguez, Sonia ; Postigo, Jorge; Merino, Jesús; Merino, Ramón ; Zubiaur, Mercedes; Sancho, Jaime
Issue Date2014
CitationHUPO (2014)
Abstract[Introduction and objectives]: Collagen type II-induced arthritis (CIA) is an autoimmune disease that is accompanied by a complex host systemic response, which includes inflammatory and autoimmune reactions. Since to develop CIA is required the injection of antigen in complete Freund's adjuvant (CFA), which is a water-in-mineral-oil emulsion containing killed Mycobacteria, systemic response proteins related with inflammation can confound the detection or diagnosis of arthritis. CIA in CD38 deficient mice (CD38 KO) is milder than that in C57BL/6 (B6 WT) mice. The purpose of this study was to identify CD38-dependent changes in serum protein abundance in mice with CIA versus mice with overt inflammation caused by CFA injection alone. [Methods]: Blood serum samples were treated with ProteoMiner beads to equalize protein concentrations and subjected to 2D-DiGE and MS-MALDI-TOF/TOF analysis to identify proteins that were differentially expressed in CD38 KO and B6 WT mice with arthritis or with inflammation. Differential protein expression was validated by ELISA, or Western-blotting. [Results and Discussion]: Altered proteins included those involved in acute phase response (SAA1), inflammation (B2m), complement activation (Ficolins, C4-B fragments, C1qb, C3 chain) and lipid metabolism (ApoE, ApoAI, ApoAII and ApoJ/Clusterin). Multivariate analyses of serum protein profiles between the autoimmune and inflammation models revealed a set of proteins that were distinct to arthritis-bearing mice, whereas other protein changes were clearly part of the non-specific host inflammatory response. Moreover, the proteins that were differentially expressed were useful to discriminate between CD38 KO and B6 WT mice in their response to either collagen immunization or to CFA injection. [Conclusions]: This proteomic approach provides a basis for distinguishing between protein changes in serum or plasma that are arthritis-related and those that are part of a non-specific host inflammatory response.
DescriptionResumen del trabajo presentado al 13th Human Proteome Organization World Congress celebrado en Madrid (España) del 5 al 8 de octubre de 2014.
Appears in Collections:(IBBTEC) Comunicaciones congresos
(IPBLN) Comunicaciones congresos
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