Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/130576
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | H-Ras distribution and signaling in plasma membrane microdomains are regulated by acylation and deacylation events |
Autor: | Agudo-Ibáñez, Lorena CSIC; Herrero, Ana CSIC ORCID; Barbacid, Mariano CSIC; Crespo, Piero CSIC ORCID | Fecha de publicación: | 2015 | Editor: | American Society for Microbiology | Citación: | Molecular and Cellular Biology 35(11): 1898-1914 (2015) | Resumen: | H-Ras must adhere to the plasma membrane to be functional. This is accomplished by posttranslational modifications, including palmitoylation, a reversible process whereby H-Ras traffics between the plasma membrane and the Golgi complex. At the plasma membrane, H-Ras has been proposed to occupy distinct sublocations, depending on its activation status: lipid rafts/detergent- resistant membrane fractions when bound to GDP, diffusing to disordered membrane/soluble fractions in response to GTP loading. Herein, we demonstrate that H-Ras sublocalization is dictated by its degree of palmitoylation in a cell type-specific manner. Whereas H-Ras localizes to detergent-resistant membrane fractions in cells with low palmitoylation activity, it locates to soluble membrane fractions in lineages where it is highly palmitoylated. Interestingly, in both cases GTP loading results in H-Ras diffusing away from its original sublocalization. Moreover, tilting the equilibrium between palmitoylation and depalmitoylation processes can substantially alter H-Ras segregation and, subsequently, its biochemical and biological functions. Thus, the palmitoylation/depalmitoylation balance not only regulates H-Ras cycling between endomembranes and the plasma membrane but also serves as a key orchestrator of H-Ras lateral diffusion between different types of plasma membrane and thereby of H-Ras signaling. | URI: | http://hdl.handle.net/10261/130576 | DOI: | 10.1128/MCB.01398-14 | Identificadores: | doi: 10.1128/MCB.01398-14 issn: 1098-5549 e-issn: 0270-7306 |
Aparece en las colecciones: | (IBBTEC) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
16
checked on 20-mar-2024
SCOPUSTM
Citations
28
checked on 16-abr-2024
WEB OF SCIENCETM
Citations
28
checked on 29-feb-2024
Page view(s)
246
checked on 21-abr-2024
Download(s)
91
checked on 21-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.