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Title

Negative feedback and transcriptional overshooting in a regulatory network for horizontal gene transfer

AuthorsFernández-López, Raul; Campo, Irene del ; Revilla, Carlos ; Cuevas, Ana ; Cruz, Fernando de la
Issue Date2014
PublisherPublic Library of Science
CitationPLoS Genetics 10(2): e1004171 (2014)
AbstractHorizontal gene transfer (HGT) is a major force driving bacterial evolution. Because of their ability to cross inter-species barriers, bacterial plasmids are essential agents for HGT. This ability, however, poses specific requisites on plasmid physiology, in particular the need to overcome a multilevel selection process with opposing demands. We analyzed the transcriptional network of plasmid R388, one of the most promiscuous plasmids in Proteobacteria. Transcriptional analysis by fluorescence expression profiling and quantitative PCR revealed a regulatory network controlled by six transcriptional repressors. The regulatory network relied on strong promoters, which were tightly repressed in negative feedback loops. Computational simulations and theoretical analysis indicated that this architecture would show a transcriptional burst after plasmid conjugation, linking the magnitude of the feedback gain with the intensity of the transcriptional burst. Experimental analysis showed that transcriptional overshooting occurred when the plasmid invaded a new population of susceptible cells. We propose that transcriptional overshooting allows genome rebooting after horizontal gene transfer, and might have an adaptive role in overcoming the opposing demands of multilevel selection.
DescriptionThis is an open-access article distributed under the terms of the Creative Commons Attribution License.
Publisher version (URL)http://dx.doi.org/10.1371/journal.pgen.1004171
URIhttp://hdl.handle.net/10261/130438
DOI10.1371/journal.pgen.1004171
Identifiersdoi: 10.1371/journal.pgen.1004171
issn: 1553-7390
e-issn: 1553-7404
Appears in Collections:(IBBTEC) Artículos
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