English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/130171
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Maps of lamin and emerin associated chromatin domains in C. elegans reveal global similarities and unique local enrichments

AuthorsGonzález-Aguilera, Cristina ; Ikegami, Kohta; Luis, Alberto de ; Íñiguez, María; Cabello, Juan ; Lieb, Jason D.; Askjaer, Peter
Issue Date2013
Citation4th Spanish Worm Meeting (2013)
AbstractThe nuclear envelope (NE) is an essential lipo-protein structure in the eukaryotic cell. Besides its role as a fundamental barrier between the nucleus and the cytoplasm, it has more recently been revealed as a dynamic structure that plays an important role in the regulation of gene expression and chromatin organization. Traditionally, the NE has been associated with heterochromatin and silent DNA. However, recent studies have shown that there is also active chromatin at the nuclear periphery and genes that change localization depending on their transcriptional state. Despite the efforts realized, the underlying molecular mechanisms are not well understood. To characterize the association of chromatin with the NE in an intact organism, we performed genome-wide analyses using DamID and RNA-seq in the nematode Caenorhabditis elegans. DamID is based on the expression in vivo of chimeric proteins consisting of DNA adenine methyltransferase (Dam) from E. coli fused to a chromatin-interacting protein; Dam thus methylates the DNA in the vicinity of the native binding sites. We have created C. elegans strains containing single copy insertions of Dam fused to two NE proteins, lamin/LMN-1 and emerin/EMR-1. As expected, the global chromatin association profiles are very similar for the two proteins, but each protein shows unique enrichments at local domains. Employing a genetically amenable model system enables us to analyze nuclear architecture across several NE mutant backgrounds, and through different developmental stages. We have also performed RNA-seq analysis in wild type, emr-1 and lem-2 mutants to correlate the NE association of genes with their transcriptional status. These experiments suggest that both features are robust against loss of single NE proteins since effects are mainly observed on a local scale. In contrast, simultaneous depletion of EMR-1 and LEM-2 produces more dramatic effects, indicative of redundant functions in nuclear organization and gene expression.
DescriptionResumen del trabajo presentado al 4th Spanish Worm Meeting, celebrado en Carmona (Sevilla) del 14 al 15 de marzo de 2013.
Appears in Collections:(CABD) Comunicaciones congresos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.