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dc.contributor.authorOjeda, Edilbertoes_ES
dc.contributor.authorPuras, Gustavoes_ES
dc.contributor.authorAgirre, Mireiaes_ES
dc.contributor.authorZárate, Jones_ES
dc.contributor.authorGrijalvo, Santiagoes_ES
dc.contributor.authorPons Pons, Ramónes_ES
dc.contributor.authorEritja Casadellà, Ramónes_ES
dc.contributor.authorMartínez Navarrete, Gema C.es_ES
dc.contributor.authorSoto-Sánchez, Cristinaes_ES
dc.contributor.authorFernández, E.es_ES
dc.contributor.authorPedraz, José Luíses_ES
dc.date.accessioned2016-03-08T09:17:23Z-
dc.date.available2016-03-08T09:17:23Z-
dc.date.issued2015-04-14-
dc.identifier.citationOrganic and Biomolecular Chemistryes_ES
dc.identifier.urihttp://hdl.handle.net/10261/129864-
dc.description.abstractWe designed niosomes based on three lipids that differed only in the polar-head group to analyze their influence on the transfection efficiency. These lipids were characterized by small-angle X-ray scattering before being incorporated into the niosomes which were characterized in terms of pKa, size, zeta potential, morphology and physical stability. Nioplexes were obtained upon the addition of a plasmid. Different ratios (w/w) were selected to analyze the influence of this parameter on size, charge and the ability to condense, release and protect the DNA. In vitro transfection experiments were performed in HEK-293, ARPE-19 and MSC-D1 cells. Our results show that the chemical composition of the cationic head-group clearly affects the physicochemical parameters of the niosomes and especially the transfection efficiency. Only niosomes based on cationic lipids with a dimethyl amino head group (lipid 3) showed a transfection capacity when compared with their counterparts amino (lipid 1) and tripeptide head-groups (lipid 2). Regarding cell viability, we clearly observed that nioplexes based on the cationic lipid 3 had a more deleterious effect than their counterparts, especially in ARPE-19 cells at 20/1 and 30/1 ratios. Similar studies could be extended to other series of cationic lipids in order to progress in the research on safe and efficient non-viral vectors for gene delivery purposes.es_ES
dc.description.sponsorshipThis project was partially supported by the University of the Basque Country UPV/EHU (UFI 11/32), the National Council of Science and Technology (CONAYT), Mexico, Reg. # 217101, the Spanish Ministry of Education (Grant CTQ2010-20541, CTQ2010-14897), the Basque Government (Department of Education, University and Research, predoctoral BFI-2011-2226 grant), the Generalitat de Catalunya (2009SGR208, 2009SGR1331) and the Instituto de Salud Carlos III. Technical and human support provided by SGIker (UPV/EHU) is gratefully acknowledged. Authors also wish to thank the intellectual and technical assistance from the platform for Drug Formulation (NANBIOSIS) CIBER-BBN.es_ES
dc.language.isoenges_ES
dc.publisherRoyal Society of Chemistry (UK)es_ES
dc.relation.isversionofPostprintes_ES
dc.rightsopenAccessen_EN
dc.subjectCellses_ES
dc.subjectGeneses_ES
dc.subjectMolecular biologyes_ES
dc.subjectgenetic transfectiones_ES
dc.subjectLipidses_ES
dc.titleNiosomes based on synthetic cationic lipids for gene delivery: The influence of polar head-groups on the transfection efficiency in HEK-293, ARPE-19 and MSC-D1 cellses_ES
dc.typeartículoes_ES
dc.identifier.doi10.1039/c4ob02087a-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://pubs.rsc.org/en/content/articlelanding/2015/ob/c4ob02087a#!divAbstractes_ES
dc.embargo.terms2016-04-15es_ES
dc.rights.licensehttp://www.sherpa.ac.uk/romeo/issn/1477-0520/es_ES
dc.relation.csices_ES
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
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