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http://hdl.handle.net/10261/129864
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dc.contributor.author | Ojeda, Edilberto | es_ES |
dc.contributor.author | Puras, Gustavo | es_ES |
dc.contributor.author | Agirre, Mireia | es_ES |
dc.contributor.author | Zárate, Jon | es_ES |
dc.contributor.author | Grijalvo, Santiago | es_ES |
dc.contributor.author | Pons Pons, Ramón | es_ES |
dc.contributor.author | Eritja Casadellà, Ramón | es_ES |
dc.contributor.author | Martínez Navarrete, Gema C. | es_ES |
dc.contributor.author | Soto-Sánchez, Cristina | es_ES |
dc.contributor.author | Fernández, E. | es_ES |
dc.contributor.author | Pedraz, José Luís | es_ES |
dc.date.accessioned | 2016-03-08T09:17:23Z | - |
dc.date.available | 2016-03-08T09:17:23Z | - |
dc.date.issued | 2015-04-14 | - |
dc.identifier.citation | Organic and Biomolecular Chemistry | es_ES |
dc.identifier.uri | http://hdl.handle.net/10261/129864 | - |
dc.description.abstract | We designed niosomes based on three lipids that differed only in the polar-head group to analyze their influence on the transfection efficiency. These lipids were characterized by small-angle X-ray scattering before being incorporated into the niosomes which were characterized in terms of pKa, size, zeta potential, morphology and physical stability. Nioplexes were obtained upon the addition of a plasmid. Different ratios (w/w) were selected to analyze the influence of this parameter on size, charge and the ability to condense, release and protect the DNA. In vitro transfection experiments were performed in HEK-293, ARPE-19 and MSC-D1 cells. Our results show that the chemical composition of the cationic head-group clearly affects the physicochemical parameters of the niosomes and especially the transfection efficiency. Only niosomes based on cationic lipids with a dimethyl amino head group (lipid 3) showed a transfection capacity when compared with their counterparts amino (lipid 1) and tripeptide head-groups (lipid 2). Regarding cell viability, we clearly observed that nioplexes based on the cationic lipid 3 had a more deleterious effect than their counterparts, especially in ARPE-19 cells at 20/1 and 30/1 ratios. Similar studies could be extended to other series of cationic lipids in order to progress in the research on safe and efficient non-viral vectors for gene delivery purposes. | es_ES |
dc.description.sponsorship | This project was partially supported by the University of the Basque Country UPV/EHU (UFI 11/32), the National Council of Science and Technology (CONAYT), Mexico, Reg. # 217101, the Spanish Ministry of Education (Grant CTQ2010-20541, CTQ2010-14897), the Basque Government (Department of Education, University and Research, predoctoral BFI-2011-2226 grant), the Generalitat de Catalunya (2009SGR208, 2009SGR1331) and the Instituto de Salud Carlos III. Technical and human support provided by SGIker (UPV/EHU) is gratefully acknowledged. Authors also wish to thank the intellectual and technical assistance from the platform for Drug Formulation (NANBIOSIS) CIBER-BBN. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Royal Society of Chemistry (UK) | es_ES |
dc.relation.isversionof | Postprint | es_ES |
dc.rights | openAccess | en_EN |
dc.subject | Cells | es_ES |
dc.subject | Genes | es_ES |
dc.subject | Molecular biology | es_ES |
dc.subject | genetic transfection | es_ES |
dc.subject | Lipids | es_ES |
dc.title | Niosomes based on synthetic cationic lipids for gene delivery: The influence of polar head-groups on the transfection efficiency in HEK-293, ARPE-19 and MSC-D1 cells | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1039/c4ob02087a | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://pubs.rsc.org/en/content/articlelanding/2015/ob/c4ob02087a#!divAbstract | es_ES |
dc.embargo.terms | 2016-04-15 | es_ES |
dc.rights.license | http://www.sherpa.ac.uk/romeo/issn/1477-0520/ | es_ES |
dc.relation.csic | Sí | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.grantfulltext | open | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
Aparece en las colecciones: | (IQAC) Artículos |
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OBC2015_Niosomes.pdf | 1,48 MB | Adobe PDF | Visualizar/Abrir |
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