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dc.contributor.author | Marrero, Patricia | en_US |
dc.contributor.author | Pérez Padilla, David | en_US |
dc.contributor.author | Valdés, Francisco | en_US |
dc.contributor.author | Nogales, Manuel | - |
dc.date.accessioned | 2009-05-12T06:18:13Z | - |
dc.date.available | 2009-05-12T06:18:13Z | - |
dc.date.issued | 2007-01-12 | en_US |
dc.identifier.citation | Chemoecology 17(1): 47-50 (2007) | en_US |
dc.identifier.issn | 0937-7409 | - |
dc.identifier.uri | http://hdl.handle.net/10261/12983 | - |
dc.description | 4 pages, 1 table.-- Printed version published Mar 2007. | - |
dc.description.abstract | Three chemical viability tests were evaluated in the seed dispersal system of Rubia fruticosa, in which three main groups of dispersers participate: reptiles, birds and mammals. Tetrazolium chloride (TTC) and indigo carmine (IC) indicated a lower viability of seeds from droppings of introduced rabbits (Oryctolagus cuniculus) than of those from control plants and the native dispersers, lizards and gulls. In the rabbit seed treatment, significant differences were observed between results obtained with TTC and IC tests. Interpretation of these data, due to the presence of doubtful embryo staining, was more difficult using the IC test. Furthermore, some seeds that were clearly dead had been underestimated. In contrast with results obtained from the two staining methods, the EC test did not confirm that viability of control seeds and those seeds consumed by native dispersers were clearly higher than in seeds ingested by O. cuniculus. Further, compared to the other two tests, the EC method requires more careful handling of the embryo during the extraction process to avoid errors in viability estimation, since this method measures concentration of electrolytes that are released through cellular membranes. Thus, TTC was the most reliable test to assess seed viability in the seed dispersal system of R. fruticosa, and these results agree with those obtained in previous germination experiments made on the same set of seeds given the same treatments. | - |
dc.description.abstract | Three chemical viability tests were evaluated in the seed dispersal system of Rubia fruticosa, in which three main groups of dispersers participate: reptiles, birds and mammals. Tetrazolium chloride (TTC) and indigo carmine (IC) indicated a lower viability of seeds from droppings of introduced rabbits (Oryctolagus cuniculus) than of those from control plants and the native dispersers, lizards and gulls. In the rabbit seed treatment, significant differences were observed between results obtained with TTC and IC tests. Interpretation of these data, due to the presence of doubtful embryo staining, was more difficult using the IC test. Furthermore, some seeds that were clearly dead had been underestimated. In contrast with results obtained from the two staining methods, the EC test did not confirm that viability of control seeds and those seeds consumed by native dispersers were clearly higher than in seeds ingested by O. cuniculus. Further, compared to the other two tests, the EC method requires more careful handling of the embryo during the extraction process to avoid errors in viability estimation, since this method measures concentration of electrolytes that are released through cellular membranes. Thus, TTC was the most reliable test to assess seed viability in the seed dispersal system of R. fruticosa, and these results agree with those obtained in previous germination experiments made on the same set of seeds given the same treatments. | - |
dc.description.sponsorship | This research was supported by grants to Patricia Marrero and David P. Padilla from the Proyectos Intramurales Especiales, 2004 3 0E 169 of the Spanish National Scientific Research Council (Consejo Superior de Investigaciones Científicas) and the Canarian Government, respectively. It was partially financed by the projects BOS2001-0610 (Ministerio de Educación y Ciencia) and PI042004/037 (Consejería de Educación, Cultura y Deportes, Gobierno de Canarias), which are partially supported by Feder funds from the European Union. | - |
dc.format.extent | 2373 bytes | - |
dc.format.extent | 143435 bytes | - |
dc.format.mimetype | text/plain | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | en_US |
dc.publisher | Springer Nature | - |
dc.rights | closedAccess | en_US |
dc.subject | Tetrazolium | - |
dc.subject | Indigo carmine | - |
dc.subject | Electroconductivity | - |
dc.subject | Macaronesian Islands | - |
dc.subject | Rubia fruticosa | - |
dc.subject | Vertebrate dispersers | - |
dc.title | Comparison of three chemical tests to assess seed viability: the seed dispersal system of the Macaronesian endemic plant Rubia fruticosa (Rubiaceae) as an example | en_US |
dc.type | artículo | en_US |
dc.identifier.doi | 10.1007/s00049-006-0360-x | - |
dc.relation.publisherversion | http://dx.doi.org/10.1007/s00049-006-0360-x | - |
dc.identifier.e-issn | 1423-0445 | - |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas (España) | - |
dc.contributor.funder | Gobierno de Canarias | - |
dc.contributor.funder | Ministerio de Educación y Ciencia (España) | - |
dc.contributor.funder | European Commission | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003339 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
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