Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/12983
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

Comparison of three chemical tests to assess seed viability: the seed dispersal system of the Macaronesian endemic plant Rubia fruticosa (Rubiaceae) as an example

AutorMarrero, Patricia CSIC ORCID ; Pérez Padilla, David CSIC; Valdés, Francisco; Nogales, Manuel CSIC ORCID
Palabras claveTetrazolium
Indigo carmine
Electroconductivity
Macaronesian Islands
Rubia fruticosa
Vertebrate dispersers
Fecha de publicación12-ene-2007
EditorSpringer Nature
CitaciónChemoecology 17(1): 47-50 (2007)
ResumenThree chemical viability tests were evaluated in the seed dispersal system of Rubia fruticosa, in which three main groups of dispersers participate: reptiles, birds and mammals. Tetrazolium chloride (TTC) and indigo carmine (IC) indicated a lower viability of seeds from droppings of introduced rabbits (Oryctolagus cuniculus) than of those from control plants and the native dispersers, lizards and gulls. In the rabbit seed treatment, significant differences were observed between results obtained with TTC and IC tests. Interpretation of these data, due to the presence of doubtful embryo staining, was more difficult using the IC test. Furthermore, some seeds that were clearly dead had been underestimated. In contrast with results obtained from the two staining methods, the EC test did not confirm that viability of control seeds and those seeds consumed by native dispersers were clearly higher than in seeds ingested by O. cuniculus. Further, compared to the other two tests, the EC method requires more careful handling of the embryo during the extraction process to avoid errors in viability estimation, since this method measures concentration of electrolytes that are released through cellular membranes. Thus, TTC was the most reliable test to assess seed viability in the seed dispersal system of R. fruticosa, and these results agree with those obtained in previous germination experiments made on the same set of seeds given the same treatments.
Three chemical viability tests were evaluated in the seed dispersal system of Rubia fruticosa, in which three main groups of dispersers participate: reptiles, birds and mammals. Tetrazolium chloride (TTC) and indigo carmine (IC) indicated a lower viability of seeds from droppings of introduced rabbits (Oryctolagus cuniculus) than of those from control plants and the native dispersers, lizards and gulls. In the rabbit seed treatment, significant differences were observed between results obtained with TTC and IC tests. Interpretation of these data, due to the presence of doubtful embryo staining, was more difficult using the IC test. Furthermore, some seeds that were clearly dead had been underestimated. In contrast with results obtained from the two staining methods, the EC test did not confirm that viability of control seeds and those seeds consumed by native dispersers were clearly higher than in seeds ingested by O. cuniculus. Further, compared to the other two tests, the EC method requires more careful handling of the embryo during the extraction process to avoid errors in viability estimation, since this method measures concentration of electrolytes that are released through cellular membranes. Thus, TTC was the most reliable test to assess seed viability in the seed dispersal system of R. fruticosa, and these results agree with those obtained in previous germination experiments made on the same set of seeds given the same treatments.
Descripción4 pages, 1 table.-- Printed version published Mar 2007.
Versión del editorhttp://dx.doi.org/10.1007/s00049-006-0360-x
URIhttp://hdl.handle.net/10261/12983
DOI10.1007/s00049-006-0360-x
ISSN0937-7409
E-ISSN1423-0445
Aparece en las colecciones: (IPNA) Artículos




Ficheros en este ítem:
Fichero Descripción Tamaño Formato
Comp-Nogales-2007-Chemoecology.pdfArtículo principal185,83 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo

CORE Recommender

SCOPUSTM   
Citations

19
checked on 27-mar-2024

WEB OF SCIENCETM
Citations

20
checked on 20-feb-2024

Page view(s)

354
checked on 28-mar-2024

Download(s)

170
checked on 28-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.