Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/129549COMPARTIR / EXPORTAR:
 SHARE
 CORE
BASE
|
|
| Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
| Título: | Sox2, Tlx, Gli3, and Her9 converge on Rx2 to define retinal stem cells in vivo |
Autor: | Centanin, Lázaro; Wittbrodt, Beate; Martínez-Morales, Juan Ramón CSIC ORCID; Wittbrodt, Joachim | Palabras clave: | Transcriptional network Retinal stem cells Neural stem cells De-differentiation Gene regulation |
Fecha de publicación: | 2015 | Editor: | Nature Publishing Group | Citación: | EMBO Journal 34(11): 1572-1588 (2015) | Resumen: | Transcriptional networks defining stemness in adult neural stem cells (NSCs) are largely unknown. We used the proximal cis-regulatory element (pCRE) of the retina-specific homeobox gene 2 (rx2) to address such a network. Lineage analysis in the fish retina identified rx2 as marker for multipotent NSCs. rx2-positive cells located in the peripheral ciliary marginal zone behave as stem cells for the neuroretina, or the retinal pigmented epithelium. We identified upstream regulators of rx2 interrogating the rx2 pCRE in a trans-regulation screen and focused on four TFs (Sox2, Tlx, Gli3, and Her9) activating or repressing rx2 expression. We demonstrated direct interaction of the rx2 pCRE with the four factors in vitro and in vivo. By conditional mosaic gain- and loss-of-function analyses, we validated the activity of those factors on regulating rx2 transcription and consequently modulating neuroretinal and RPE stem cell features. This becomes obvious by the rx2-mutant phenotypes that together with the data presented above identify rx2 as a transcriptional hub balancing stemness of neuroretinal and RPE stem cells in the adult fish retina. | Descripción: | This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 License.-- et al. | Versión del editor: | http://dx.doi.org/10.15252/embj.201490706 | URI: | http://hdl.handle.net/10261/129549 | DOI: | 10.15252/embj.201490706 | Identificadores: | doi: 10.15252/embj.201490706 e-issn: 1460-2075 issn: 0261-4189 |
| Aparece en las colecciones: | (CABD) Artículos |
Ficheros en este ítem:
| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| Sox2TlxGli3,.pdf | 4,12 MB | Adobe PDF |  Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
36
checked on 13-abr-2024
SCOPUSTM
Citations
49
checked on 19-abr-2024
WEB OF SCIENCETM
Citations
48
checked on 22-feb-2024
Page view(s)
274
checked on 23-abr-2024
Download(s)
353
checked on 23-abr-2024
