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Title

Chromatids segregate without centrosomes during Caenorhabditis elegans mitosis in a Ran- and CLASP-dependent manner

AuthorsNahaboo, Wallis; Zouak, Melissa; Askjaer, Peter ; Delattre, Marie
Issue Date2015
PublisherAmerican Society for Cell Biology
CitationMolecular Biology of the Cell 26(11): 2020-2029 (2015)
AbstractDuring mitosis, chromosomes are connected to a microtubule-based spindle. Current models propose that displacement of the spindle poles and/or the activity of kinetochore microtubules generate mechanical forces that segregate sister chromatids. Using laser destruction of the centrosomes during Caenorhabditis elegans mitosis, we show that neither of these mechanisms is necessary to achieve proper chromatid segregation. Our results strongly suggest that an outward force generated by the spindle midzone, independently of centrosomes, is sufficient to segregate chromosomes in mitotic cells. Using mutant and RNAi analysis, we show that the microtubule-bundling protein SPD-1/MAP-65 and BMK-1/kinesin-5 act as a brake opposing the force generated by the spindle midzone. Conversely, we identify a novel role for two microtubule-growth and nucleation agents, Ran and CLASP, in the establishment of the centrosome-independent force during anaphase. Their involvement raises the interesting possibility that microtubule polymerization of midzone microtubules is continuously required to sustain chromosome segregation during mitosis.
DescriptionThis article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
Publisher version (URL)http://dx.doi.org/10.1091/mbc.E14-12-1577
URIhttp://hdl.handle.net/10261/129463
DOI10.1091/mbc.E14-12-1577
Identifiersdoi: 10.1091/mbc.E14-12-1577
e-issn: 1939-4586
issn: 1059-1524
Appears in Collections:(CABD) Artículos
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