Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/129204
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

Inorganic pyrophosphatase defects lead to cell cycle arrest and autophagic cell death through NAD+ depletion in fermenting yeast

AutorSerrano-Bueno, Gloria CSIC ORCID ; Hernández, Agustín CSIC ORCID; López-Lluch, Guillermo CSIC ORCID CVN ; Pérez-Castiñeira, J. R. CSIC ORCID ; Navas, Plácido CSIC ORCID; Serrano, Aurelio CSIC ORCID
Palabras claveAutophagy
Cell death
Energy metabolism
NAD
Yeast metabolism
Fecha de publicación2013
EditorAmerican Society for Biochemistry and Molecular Biology
CitaciónJournal of Biological Chemistry 288(18): 13082-13092 (2013)
ResumenInorganic pyrophosphatases are required for anabolism to take place in all living organisms. Defects in genes encoding these hydrolytic enzymes are considered inviable, although their exact nature has not been studied at the cellular and molecular physiology levels. Using a conditional mutant in IPP1, the Saccharomyces cerevisiae gene encoding the cytosolic soluble pyrophosphatase, we show that respiring cells arrest in S phase upon Ipp1p deficiency, but they remain viable and resume growth if accumulated pyrophosphate is removed. However, fermenting cells arrest in G1/G0 phase and suffer massive vacuolization and eventual cell death by autophagy. Impaired NAD(+) metabolism is a major determinant of cell death in this scenario because demise can be avoided under conditions favoring accumulation of the oxidized pyridine coenzyme. These results posit that the mechanisms related to excess pyrophosphate toxicity in eukaryotes are dependent on the energy metabolism of the cell.
Versión del editorhttp://dx.doi.org/10.1074/jbc.M112.439349
URIhttp://hdl.handle.net/10261/129204
DOI10.1074/jbc.M112.439349
ISSN0021-9258
E-ISSN1083-351X
Aparece en las colecciones: (IBVF) Artículos
(CABD) Artículos




Ficheros en este ítem:
Fichero Descripción Tamaño Formato
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo

CORE Recommender

PubMed Central
Citations

21
checked on 21-mar-2024

SCOPUSTM   
Citations

32
checked on 24-mar-2024

WEB OF SCIENCETM
Citations

32
checked on 24-feb-2024

Page view(s)

307
checked on 27-mar-2024

Download(s)

99
checked on 27-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.