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dc.contributor.authorRodríguez-Sánchez, Soniaes_ES
dc.contributor.authorGarcía-Sarrió, María Jesúses_ES
dc.contributor.authorQuintanilla-López, Jesús Eduardoes_ES
dc.contributor.authorSoria, Ana C.es_ES
dc.contributor.authorSanz, M. Luzes_ES
dc.date.issued2015-
dc.identifier.citationJournal of Chromatography A 1423: 104-110 (2015)es_ES
dc.identifier.issn0021-9673-
dc.identifier.urihttp://hdl.handle.net/10261/129118-
dc.description.abstractA method by hydrophilic interaction liquid chromatography coupled to tandem mass spectrometry (HILIC-MS2) has been successfully developed for the simultaneous analysis of bioactive iminosugars and other low molecular weight carbohydrates in Aglaonema leaf extracts. Among other experimental chromatographic conditions, mobile phase eluents, additives and column temperature were evaluated in terms of retention time, resolution, peak width and symmetry provided for target carbohydrates. In general, narrow peaks (wh: 0.2–0.6 min) with good symmetry (As: 0.9–1.3) and excellent resolution (Rs > 1.8) were obtained for iminosugars using an acetonitrile:water gradient with 5 mM ammonium acetate in both eluents at 55 °C. Tandem mass spectra were used to confirm the presence of previously detected iminosugars in Aglaonema extracts and to tentatively identify for the first time others such as miglitol isomer, glycosyl–miglitol isomers and glycosyl–DMDP isomers. Concentration of total iminosugars varied from 1.35 to 2.84 mg g−1 in the extracts of the different Aglaonema samples analyzed. To the best of our knowledge, this is the first time that a HILIC-MS2 method has been proposed for the simultaneous analysis of iminosugars and other low molecular weight carbohydrates of Aglaonema sp. extracts.es_ES
dc.description.sponsorshipCTQ2012-32957es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relationS2013/ABI-3028/AVANSECAL-
dc.rightsclosedAccesses_ES
dc.subjectIminosugarses_ES
dc.subjectLow molecular weight carbohydrateses_ES
dc.subjectAglaonema extractses_ES
dc.subjectHydrophilic interaction liquid chromatographyes_ES
dc.subjectTandem mass spectrometryes_ES
dc.titleAnalysis of iminosugars and other low molecular weight carbohydrates in Aglaonema sp. extracts by hydrophilic interaction liquid chromatography coupled to mass spectrometryes_ES
dc.typeartículoes_ES
dc.identifier.doihttp://dx.doi.org/10.1016/j.chroma.2015.10.081-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.chroma.2015.10.081es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderComunidad de Madrides_ES
dc.contributor.funderEuropean Commissiones_ES
dc.contributor.funderFundación Ramón Areceses_ES
dc.relation.csices_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/100008054es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/100012818es_ES
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