Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/129114
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Step-wise methylation of histone H3K9 positions heterochromatin at the nuclear periphery |
Autor: | Towbin, Benjamin D.; González-Aguilera, Cristina CSIC; Sack, Ragna; Gaidatzis, Dimos; Kalck, Véronique; Meister, Peter; Askjaer, Peter CSIC ORCID ; Gasser, Susan M. | Fecha de publicación: | 2012 | Editor: | Elsevier | Citación: | Cell 150(5): 934-947 (2012) | Resumen: | The factors that sequester transcriptionally repressed heterochromatin at the nuclear periphery are currently unknown. In a genome-wide RNAi screen, we found that depletion of S-adenosylmethionine (SAM) synthetase reduces histone methylation globally and causes derepression and release of heterochromatin from the nuclear periphery in Caenorhabditis elegans embryos. Analysis of histone methyltransferases (HMTs) showed that elimination of two HMTs, MET-2 and SET-25, mimics the loss of SAM synthetase, abrogating the perinuclear attachment of heterochromatic transgenes and of native chromosomal arms rich in histone H3 lysine 9 methylation. The two HMTs target H3K9 in a consecutive fashion: MET-2, a SETDB1 homolog, mediates mono- and dimethylation, and SET-25, a previously uncharacterized HMT, deposits H3K9me3. SET-25 colocalizes with its own product in perinuclear foci, in a manner dependent on H3K9me3, but not on its catalytic domain. This colocalization suggests an autonomous, self-reinforcing mechanism for the establishment and propagation of repeat-rich heterochromatin. | Versión del editor: | http://dx.doi.org/10.1016/j.cell.2012.06.051 | URI: | http://hdl.handle.net/10261/129114 | DOI: | 10.1016/j.cell.2012.06.051 | ISSN: | 0092-8674 | E-ISSN: | 1097-4172 |
Aparece en las colecciones: | (CABD) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Step-wise methylation.pdf | 1,71 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
422
checked on 20-abr-2024
WEB OF SCIENCETM
Citations
403
checked on 26-feb-2024
Page view(s)
370
checked on 24-abr-2024
Download(s)
600
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.