English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/129007
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


DNA Topology and Global Architecture of Point Centromeres

AuthorsDíaz-Ingelmo, Ofelia ; Martínez-García, Belén ; Segura, Joana ; Valdés, Antonio ; Roca, Joaquim
Issue Date27-Oct-2015
CitationCell Reports 13(4): 667-677 (2015)
AbstractDNA is wrapped in a left-handed fashion around histone octasomes containing the centromeric histone H3 variant CENP-A. However, DNA topology studies have suggested that DNA is wrapped in a righthanded manner around the CENP-A nucleosome that occupies the yeast point centromere. Here, we determine the DNA linking number difference (DLk) stabilized by the yeast centromere and the contribution of the centromere determining elements (CDEI, CDEII, and CDEIII). We show that the intrinsic architecture of the yeast centromere stabilizes +0.6 units of DLk. This topology depends on the integrity of CDEII and CDEIII, but it is independent of cbf1 binding to CDEI and of the variable length of CDEII. These findings suggest that the interaction of the CBF3 complex with CDEIII and a distal CDEII segment configures a right-handed DNA loop that excludes CDEI. This loop is then occupied by a CENP-A histone complex, which does not have to be inherently right-handed.
Publisher version (URL)http://dx.doi.org/10.1016/j.celrep.2015.09.039
Identifiersdoi: 10.1016/j.celrep.2015.09.039
issn: 2211-1247
Appears in Collections:(IBMB) Artículos
Files in This Item:
File Description SizeFormat 
Diaz-Ingelmo-Cell-Reports-2015-v13-p667.pdf2,91 MBUnknownView/Open
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.