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Title

Transcription factor-based biosensors enlightened by the analyte

AuthorsFernández-López, Raul; Ruiz, Raúl ; Cruz, Fernando de la ; Moncalián, Gabriel
KeywordsBiosensors
Transcription factor
Effector
Aromatic compounds
Metal
Analyte
Issue Date1-Jul-2015
PublisherFrontiers in Bioscience Publications
CitationFrontiers in Microbiology 6: 648 (2015)
AbstractWhole cell biosensors (WCBs) have multiple applications for environmental monitoring, detecting a wide range of pollutants. WCBs depend critically on the sensitivity and specificity of the transcription factor (TF) used to detect the analyte. We describe the mechanism of regulation and the structural and biochemical properties of TF families that are used, or could be used, for the development of environmental WCBs. Focusing on the chemical nature of the analyte, we review TFs that respond to aromatic compounds (XylS-AraC, XylR-NtrC, and LysR), metal ions (MerR, ArsR, DtxR, Fur, and NikR) or antibiotics (TetR and MarR). Analyzing the structural domains involved in DNA recognition, we highlight the similitudes in the DNA binding domains (DBDs) of these TF families. Opposite to DBDs, the wide range of analytes detected by TFs results in a diversity of structures at the effector binding domain. The modular architecture of TFs opens the possibility of engineering TFs with hybrid DNA and effector specificities. Yet, the lack of a crisp correlation between structural domains and specific functions makes this a challenging task.
Publisher version (URL)http://dx.doi.org/10.3389/fmicb.2015.00648
URIhttp://hdl.handle.net/10261/128481
DOI10.3389/fmicb.2015.00648
ISSN1664-302X
Appears in Collections:(IBBTEC) Artículos
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