Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/127996
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | A quantitative 1H NMR approach for evaluating the metabolic response of Saccharomyces cerevisiae to mild heat stress |
Autor: | Puig-Castellví, Francesc CSIC ORCID; Alfonso, Ignacio CSIC ORCID ; Piña, Benjamín CSIC ORCID ; Tauler, Romà CSIC ORCID | Palabras clave: | 1H NMR Batman OSC-PLS-DA Temperatures Yeast Chemometric analysis |
Fecha de publicación: | 26-may-2015 | Editor: | Springer Nature | Citación: | Metabolomics | Resumen: | Effect of growth temperature on the yeast (Saccharomyces cerevisiae) metabolome has been analysed by one-dimensional proton NMR spectroscopy (1H NMR). Potential biomarkers have been first identified by a non-targeted chemometric evaluation of the spectra, followed by a comprehensive analysis of bayesian estimated concentrations of target metabolites in extracts of cells growth either at 30 or 37 °C. Tentative identification of metabolites whose concentrations were affected by this mild heat-shock stress was attempted by partial least squares-discriminant analysis (PLS-DA) on 1H NMR data, combined with Statistical TOtal Correlation SpectroscopY, and further confirmed with empirical data. An extensive assignment for most of the detected NMR signals was performed, with a total number of 38 identified metabolites. Concentrations estimated using automatic BATMAN modelling revealed that bayesian integration is a sufficient approach for obtaining relevant concentration changes of metabolites and biological information of interest. In contrast to when it is applied directly on spectral data, the application of PLS-DA on BATMAN recovered metabolite concentration estimates allowed for a better overview of the investigated samples, since more metabolites were highlighted in the discriminatory model. Observed changes in metabolite concentrations were consistent with the expected process of temperature acclimation, showing alterations in amino acid cellular pools, nucleotide metabolism and lipid composition. The strategy described in this work can thus be proposed as a powerful and easy tool to investigate complex biological processes, from biomarker screening and discovery to the study of metabolite network changes in biological processes. | Versión del editor: | DOI: 10.1007/s11306-015-0812-9 | URI: | http://hdl.handle.net/10261/127996 | DOI: | 10.1007/s11306-015-0812-9 |
Aparece en las colecciones: | (IDAEA) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
A Quantitative 1H NMR Approach for Evaluating the Metabolic Response of Saccharomyces cerevisiae to Mild Heat Stress.docx | 16,86 MB | Microsoft Word XML | Visualizar/Abrir | |
supplementary a quantitative.pdf | 2,85 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
22
checked on 01-abr-2024
WEB OF SCIENCETM
Citations
19
checked on 29-feb-2024
Page view(s)
367
checked on 22-abr-2024
Download(s)
239
checked on 22-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.