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Title

Identification and Structural Analysis of Amino Acid Substitutions that Increase the Stability and Activity of Aspergillus niger Glucose Oxidase

AuthorsMarín Navarro, Julia ; Roupain, Nicole; Talens Perales, David ; Polaina Molina, Julio
KeywordsGlucose oxidase
Glucose biosensors
Enzyme's thermal stability
Issue Date7-Dec-2015
PublisherPublic Library of Science
CitationPLoS One 10(12): e0144289 (2015)
AbstractGlucose oxidase is one of the most conspicuous commercial enzymes due to its many different applications in diverse industries such as food, chemical, energy and textile. Among these applications, the most remarkable is the manufacture of glucose biosensors and in particular sensor strips used to measure glucose levels in serum. The generation of ameliorated versions of glucose oxidase is therefore a significant biotechnological objective. We have used a strategy that combined random and rational approaches to isolate uncharacterized mutations of Aspergillus niger glucose oxidase with improved properties. As a result, we have identified two changes that increase significantly the enzyme's thermal stability. One (T554M) generates a sulfur-pi interaction and the other (Q90R/Y509E) introduces a new salt bridge near the interphase of the dimeric protein structure. An additional double substitution (Q124R/L569E) has no significant effect on stability but causes a twofold increase of the enzyme's specific activity. Our results disclose structural motifs of the protein which are critical for its stability. The combination of mutations in the Q90R/Y509E/T554M triple mutant yielded a version of A. niger glucose oxidase with higher stability than those previously described.
Publisher version (URL)http://dx.doi.org/10.1371/journal.pone.0144289
URIhttp://hdl.handle.net/10261/127806
DOI10.1371/journal.pone.0144289
ISSN1932-620
Appears in Collections:(IATA) Artículos
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