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Title

Biosafety test for plant growth-promoting bacteria: proposed environmental and human safety index (EHSI) protocol

AuthorsVilchez, Juan I.; Navas, Alfonso ; González-López, Jesús; Cobacho Arcos, Susana ; Manzanera, Maximino
KeywordsPlant-growth-promoting-bacteria
Inoculants
Intentional-release
Biosafety-index
Ecotoxicity
Issue Date7-Jan-2016
PublisherFrontiers in Bioscience Publications
CitationFrontiers in Microbiology 6: Article 1514 (2016)
AbstractPlant growth-promoting bacteria (PGPB) colonize plants and enhance their growth by different mechanisms. Some of these microorganisms may represent a potential threat to human, animal or plant health; however, their use might be approved in parts of Europe if they have been recommended as plant growth enhancers. The current regulatory framework has resulted in a fragmented, contradictory system, and there is an urgent need to establish harmonized protocols for the predictability, efficiency, consistency and especially the safety of PGPB for human and animal health and for the environment. In response to current efforts to update biosafety policies and provide alternative methods to replace the use of vertebrate animals, we propose a panel of tests and an evaluation system to reliably determine the biosafety of bacterial strains used as PGPB. Based on the results of different tests, we propose a scoring system to evaluate the safety of candidates for PGPB within the limitations of the assays used.
Publisher version (URL)http://dx.doi.org/10.3389/fmicb.2015.01514
URIhttp://hdl.handle.net/10261/127657
DOI10.3389/fmicb.2015.01514
ISSN1664-302X
Appears in Collections:(MNCN) Artículos
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