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dc.contributor.authorJo, Hyunbines_ES
dc.contributor.authorVentura, Marces_ES
dc.contributor.authorVidal, Nicolases_ES
dc.contributor.authorGim, Jeong-Sooes_ES
dc.contributor.authorBuchaca, Teresaes_ES
dc.contributor.authorBarmuta, L.A.es_ES
dc.contributor.authorJeppesen, Erikes_ES
dc.contributor.authorJoo, Gea-Jaees_ES
dc.date.accessioned2016-01-14T09:35:55Z-
dc.date.available2016-01-14T09:35:55Z-
dc.date.issued2016-
dc.identifier.citationEcology and Evolution : doi: 10.1002/ece3.1825 (2016)es_ES
dc.identifier.issn2045-7758-
dc.identifier.urihttp://hdl.handle.net/10261/127596-
dc.description14 páginas, 4 figuras, 5 tablases_ES
dc.description.abstractEcological monitoring contributes to the understanding of complex ecosystem functions. The diets of fish reflect the surrounding environment and habitats and may, therefore, act as useful integrating indicators of environmental status. It is, however, often difficult to visually identify items in gut contents to species level due to digestion of soft-bodied prey beyond visual recognition, but new tools rendering this possible are now becoming available. We used a molecular approach to determine the species identities of consumed diet items of an introduced generalist feeder, brown trout (Salmo trutta), in 10 Tasmanian lakes and compared the results with those obtained from visual quantification of stomach contents. We obtained 44 unique taxa (OTUs) belonging to five phyla, including seven classes, using the barcode of life approach from cytochrome oxidase I (COI). Compared with visual quantification, DNA analysis showed greater accuracy, yielding a 1.4-fold higher number of OTUs. Rarefaction curve analysis showed saturation of visually inspected taxa, while the curves from the DNA barcode did not saturate. The OTUs with the highest proportions of haplotypes were the families of terrestrial insects Formicidae, Chrysomelidae, and Torbidae and the freshwater Chironomidae. Haplotype occurrence per lake was negatively correlated with lake depth and transparency. Nearly all haplotypes were only found in one fish gut from a single lake. Our results indicate that DNA barcoding of fish diets is a useful and complementary method for discovering hidden biodiversity.es_ES
dc.description.sponsorshipThe project was funded by The Korea National Long-Term Ecological Research Project (2013), Galathea 3, Sino-Danish Centre for Education and Research (SDC) and Aarhus University (AU).es_ES
dc.language.isoenges_ES
dc.publisherJohn Wiley & Sonses_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rightsopenAccesses_ES
dc.subjectDNA barcodinges_ES
dc.subjectShallow lakeses_ES
dc.subjectFish dietes_ES
dc.subjectMonitoring tooles_ES
dc.titleDiscovering hidden biodiversity: the use of complementary monitoring of fish diet based on DNA barcoding in freshwater ecosystemses_ES
dc.typeartículoes_ES
dc.identifier.doi10.1002/ece3.1825-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1002/ece3.1825es_ES
dc.identifier.e-issn2045-7758-
dc.relation.csices_ES
dc.identifier.pmid26811787-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
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