English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/127076
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

DC FieldValueLanguage
dc.contributor.authorNieto-Domínguez, Manuel Josées_ES
dc.contributor.authorEugenio, Laura I. dees_ES
dc.contributor.authorBarriuso, Jorgees_ES
dc.contributor.authorPrieto, Aliciaes_ES
dc.contributor.authorFernández de Toro, Beatrizes_ES
dc.contributor.authorCanales, Ángeleses_ES
dc.contributor.authorMartínez, María Jesúses_ES
dc.date.issued2015-09-
dc.identifier.citationAppl Environ Microbiol 81:6380–6392 (2015)es_ES
dc.identifier.issn0099-2240-
dc.identifier.urihttp://hdl.handle.net/10261/127076-
dc.description49 p.-7 fig.-3 tab.es_ES
dc.description.abstractThis paper reports on a novel β-xylosidase from the hemicellulolytic fungus Talaromyces amestolkiae. The expression of this enzyme, called BxTW1, could be induced by beechwood xylan and was purified as a glycoprotein from culture supernatants. We characterized the gene encoding this enzyme as an intronless gene belonging to the glycoside hydrolase gene family 3 (GH3). BxTW1 exhibited transxylosylation activity in a regioselective way. This feature would allow the synthesis of oligosaccharides or other compounds not available from natural sources, such as alkyl glycosides displaying antimicrobial or surfactant properties. Regioselective transxylosylation, an uncommon combination, makes the synthesis reproducible, which is desirable for its potential industrial application. BxTW1 showed high pH stability and Cu2+ tolerance. The enzyme displayed a pI of 7.6, a molecular mass around 200 kDa in its active dimeric form, and Km and Vmax values of 0.17 mM and 52.0 U/mg, respectively, using commercial p-nitrophenyl-β-D-xylopyranoside as the substrate. The catalytic efficiencies for the hydrolysis of xylooligosaccharides were remarkably high, making it suitable for different applications in food and bioenergy industries.es_ES
dc.description.sponsorshipThis work was carried out with funding from the MINECO projects PRI-PIBAR-2011-1402 and RTC-2014-1777-3 and the Comunidad de Madrid Program RETO-PROSOT S2013/MAE-2907.es_ES
dc.language.isoenges_ES
dc.publisherAmerican Society for Microbiologyes_ES
dc.relationMINECO/ICTI2013-2016/RTC-2014-1777-3-
dc.relation.isversionofPostprintes_ES
dc.rightsopenAccessen_EN
dc.subjectLignocellulosic biomasses_ES
dc.subjectXylanes_ES
dc.subjectEnzymees_ES
dc.subjectAscomycetees_ES
dc.titleNovel pH-stable glycoside hydrolase family 3 β-xylosidase from Talaromyces amestolkiae: An enzyme displaying regioselective transxylosylationes_ES
dc.title.alternativeA new fungal B-xylosidase of biotechnological interestes_ES
dc.typeartículoes_ES
dc.identifier.doi10.1128/AEM.01744-15-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1128/AEM.01744-15es_ES
dc.identifier.e-issn1098-5336-
dc.embargo.terms2016-03es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderComunidad de Madrides_ES
dc.relation.csices_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
Nieto-Dominguez-Env. Biol.2015.pdfPostprint1,08 MBAdobe PDFThumbnail
View/Open
Show simple item record
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.