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| Título: | Electrochemical antibody-aptamer assay for VEGF cancer biomarker detection |
Autor: | Ravalli, Andrea; Marrazza, Giovanna; Rivas, Lourdes CSIC ORCID; Escosura-Muñiz, Alfredo de la CSIC ORCID; Merkoçi, Arben CSIC ORCID | Fecha de publicación: | 2014 | Editor: | Springer Nature | Citación: | Sensors and Microsystems: 175-178 (2014) | Serie: | Lecture Notes in Electrical Engineering 268 |
Resumen: | In this work, a simple and sensitive approach for VEGF detection using antibody-aptamer assay and gold screen-printed electrodes as transducers is presented. The assay was performed in a sandwich format. Anti-VEGF antibody was first chemically immobilized on the gold working electrode surface of screen-printed cell. After the incubation with the antigen, the sandwich assay was realized by incubation step with biotinylated anti-VEGF aptamer. The sensor was then incubated with streptavidin-alkaline phosphatase and with 1-naphthyl phosphate. Differential pulse voltammetry (DPV) measurements were performed to detect VEGF biomarker. | Descripción: | Proceedings of the 17th National Conference, Brescia (Italy), 5-7 February 2013. | URI: | http://hdl.handle.net/10261/126867 | DOI: | 10.1007/978-3-319-00684-0_33 | Identificadores: | doi: 10.1007/978-3-319-00684-0_33 isbn: 978-3-319-00683-3 |
| Aparece en las colecciones: | (CIN2) Libros y partes de libros |
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