Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/126442
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Diagnosing infection with small ruminant lentiviruses of genotypes A and B by combining synthetic peptides in ELISA |
Autor: | Sanjosé, Leticia CSIC; Pinczowski, Pedro; Crespo, Helena CSIC; Pérez, Marta M.; Glaria, Idoia CSIC ORCID ; Gimeno, Marina; Andrés, Damián F. de CSIC ORCID ; Amorena Zabalza, Beatriz CSIC ORCID ; Luján, Lluís; Reina, Ramsés CSIC ORCID | Palabras clave: | Small ruminant lentivirus Genotype Serology Peptide ELISA |
Fecha de publicación: | abr-2015 | Editor: | Elsevier | Citación: | Veterinary Journal 204(1): 88- 93 (2015) | Resumen: | The major challenges in diagnosing small ruminant lentivirus (SRLV) infection include early detection and genotyping of strains of epidemiological interest. A longitudinal study was carried out in Rasa Aragonesa sheep experimentally infected with viral strains of genotypes A or B from Spanish neurological and arthritic SRLV outbreaks, respectively. Sera were tested with two commercial ELISAs, three based on specific peptides and a novel combined peptide ELISA. Three different PCR assays were used to further assess infection status.The kinetics of anti-viral antibody responses were variable, with early diagnosis dependent on the type of ELISA used. Peptide epitopes of SRLV genotypes A and B combined in the same ELISA well enhanced the overall detection rate, whereas single peptides were useful for genotyping the infecting strain (A vs. B). The results of the study suggest that a combined peptide ELISA can be used for serological diagnosis of SRLV infection, with single peptide ELISAs useful for subsequent serotyping. | Versión del editor: | http://dx.doi.org/10.1016/j.tvjl.2015.01.012 | URI: | http://hdl.handle.net/10261/126442 | DOI: | 10.1016/j.tvjl.2015.01.012 | Identificadores: | e-issn: 1532-2971 issn: 1090-0233 |
Aparece en las colecciones: | (IDAB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Diagnosing infection_Sanjose.pdf | 851,2 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
14
checked on 19-abr-2024
WEB OF SCIENCETM
Citations
13
checked on 19-feb-2024
Page view(s)
396
checked on 18-abr-2024
Download(s)
452
checked on 18-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Este item está licenciado bajo una Licencia Creative Commons