Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/126061
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Metatranscriptomes reveal functional variation in diatom communities from the Antarctic Peninsula |
Autor: | Pearson, Gareth A.; Lago-Lestón, Asunción; Cánovas, Fernando; Cox, Cymon J.; Verret, Frédéric; Lasternas, Sebastien CSIC; Duarte, Carlos M. CSIC ORCID; Agustí, Susana CSIC ORCID; Serrao, Ester Álvares | Fecha de publicación: | 14-abr-2015 | Editor: | Nature Publishing Group | Citación: | ISME Journal 9(10): 2275-2289 (2015) | Resumen: | © 2015 International Society for Microbial Ecology All rights reserved. Functional genomics of diatom-dominated communities fromthe Antarctic Peninsula was studied using comparative metatranscriptomics. Samples obtained from diatom-rich communities in the Bransfield Strait, the western Weddell Sea and sea ice in the Bellingshausen Sea/Wilkins Ice Shelf yielded more than 500K pyrosequencing reads that were combined to produce a global metatranscriptome assembly. Multi-gene phylogenies recovered three distinct communities, and diatom-assigned contigs further indicated little read-sharing between communities, validating an assembly-based annotation and analysis approach. Although functional analysis recovered a core of abundant shared annotations that were expressed across the three diatom communities, over 40% of annotations (but accounting for <10% of sequences) were community-specific. The two pelagic communities differed in their expression of N-metabolism and acquisition genes, which was almost absent in post-bloom conditions in the Weddell Sea community, while enrichment of transporters for ammonia and urea in Bransfield Strait diatoms suggests a physiological stance towards acquisition of reduced N-sources. The depletion of carbohydrate and energy metabolism pathways in sea ice relative to pelagic communities, together with increased light energy dissipation (via LHCSR proteins), photorespiration, and NO<inf>3</inf> <sup>-</sup> uptake and utilization all pointed to irradiance stress and/or inorganic carbon limitation within sea ice. Ice-binding proteins and cold-shock transcription factors were also enriched in sea ice diatoms. Surprisingly, the abundance of gene transcripts for the translational machinery tracked decreasing environmental temperature across only a 4 °C range, possibly reflecting constraints on translational efficiency and protein production in cold environments. | Versión del editor: | http://dx.doi.org/10.1038/ismej.2015.40 | URI: | http://hdl.handle.net/10261/126061 | DOI: | 10.1038/ismej.2015.40 | Identificadores: | doi: 10.1038/ismej.2015.40 issn: 1751-7370 |
Aparece en las colecciones: | (IMEDEA) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
11
checked on 14-abr-2024
SCOPUSTM
Citations
34
checked on 21-abr-2024
WEB OF SCIENCETM
Citations
31
checked on 25-feb-2024
Page view(s)
261
checked on 24-abr-2024
Download(s)
80
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.