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Title

Altered glutamyl-aminopeptidase activity and expression in renal neoplasms

AuthorsBlanco, Lorena; Sanz, Begoña; Pérez, Itxaro; Sánchez, Clara E.; Candenas, M. Luz ; Pinto Pérez, Francisco M. ; Gil, Javier; Casis, Luis; López, José Ignacio; Larrinaga, Gorka
KeywordsGlutamyl-aminopeptidase
Aminopeptidase A
Angiotensinase
Angiotensin
Clear cell renal cell carcinoma
Renal neoplasm
Issue Date30-May-2014
PublisherBioMed Central
CitationBMC Cancer 14(1): 386 (2014)
Abstract[Background] Advances in the knowledge of renal neoplasms have demonstrated the implication of several proteases in their genesis, growth and dissemination. Glutamyl-aminopeptidase (GAP) (EC. 3.4.11.7) is a zinc metallopeptidase with angiotensinase activity highly expressed in kidney tissues and its expression and activity have been associated wtih tumour development.
[Methods] In this prospective study, GAP spectrofluorometric activity and immunohistochemical expression were analysed in clear-cell (CCRCC), papillary (PRCC) and chromophobe (ChRCC) renal cell carcinomas, and in renal oncocytoma (RO). Data obtained in tumour tissue were compared with those from the surrounding uninvolved kidney tissue. In CCRCC, classic pathological parameters such as grade, stage and tumour size were stratified following GAP data and analyzed for 5-year survival.
[Results] GAP activity in both the membrane-bound and soluble fractions was sharply decreased and its immunohistochemical expression showed mild staining in the four histological types of renal tumours. Soluble and membrane-bound GAP activities correlated with tumour grade and size in CCRCCs.
[Conclusions] This study suggests a role for GAP in the neoplastic development of renal tumours and provides additional data for considering the activity and expression of this enzyme of interest in the diagnosis and prognosis of renal neoplasms.
Publisher version (URL)http://dx.doi.org/10.1186/1471-2407-14-386
URIhttp://hdl.handle.net/10261/125831
DOI10.1186/1471-2407-14-386
ISSN1471-2407
Appears in Collections:(IIQ) Artículos
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