English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/12562
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Evaluation of eye and skin irritation of arginine-derivative surfactants using different in vitro endpoints as alternatives to the in vivo assays

AuthorsMartinez, V; Corsini, E; Mitjans, Montserrat; Pinazo Gassol, Aurora; Vinardell, M. Pilar
KeywordsArginine-derivative surfactant
Skin irritation
Eye irritation
Cytotoxicity
Cytokine
IL-1alpha
Issue Date15-Jul-2006
PublisherElsevier
CitationToxicology Letters 164 (3):259-267 (2006)
AbstractArginine-derivative surfactants constitute a novel class of surfactants, which can be regarded as an alternative to conventional surfactants. Prior to human exposure, it is necessary to assess their irritation potential. The classical in vivo evaluation of the irritancy potential via the Draize test has been extensively criticized. In that regard, a great number of in vitro alternatives have been developed. Erythrocytes were chosen as the target cells for eye irritation assessment and hemolysis and hemoglobin denaturation were selected as appropriate endpoints. For skin irritancy assessment, the keratinocyte cell line NCTC 2544 was used and different in vitro endpoints were measured: two cytotoxicity assays (NRU and MTT) and the synthesis of the proinflammatory cytokine IL-1alpha. The eye and skin Draize tests were also performed for comparative purposes. The results point out that, according to in vivo and in vitro assays, the new arginine-derivative surfactants have lower eye and skin irritation potential than the synthetic surfactant SDS. Furthermore, in vitro methods were also able to detect differences in irritancy among the new surfactants not noticeable by the Draize tests, indicating that in vitro methods can be more sensitive than the in vivo test, offering the opportunity to detect subtle differences in irritancy.
Description9 pages, 5 figures, 4 tables.
Publisher version (URL)http://dx.doi.org/10.1016/j.toxlet.2006.01.005
URIhttp://hdl.handle.net/10261/12562
DOI10.1016/j.toxlet.2006.01.005
ISSN0378-4274
Appears in Collections:(IQAC) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.