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Acute hypoxia produces a superoxide burst in cells

AuthorsHernansanz-Agustín, Pablo; Izquierdo-Álvarez, Alicia; Sánchez-Gómez, Francisco J. ; Lamas Peláez, Santiago ; Martínez-Ruiz, Antonio
Issue Date2014
CitationGEIRLI 2014
AbstractOxygen is a key molecule for cell metabolism. Eukaryotic cells sense the reduction in oxygen availability (hypoxia) and trigger a series of cellular and systemic responses in order to adapt to hypoxia, including the optimization of oxygen consumption. Many of these responses are mediated by a genetic programme induced by the hypoxia-inducible transcription factors (HIFs), regulated by a family of prolyl hydroxylases (PHD or EGLN) that use oxygen as a substrate producing HIF hydroxylation. In parallel to these oxygen sensors modulating gene expression within hours, acute modulation of protein function in response to hypoxia is known to occur within minutes. Free radicals acting as second messengers and oxidative post-translational modifications have been implied in both groups of responses. Localization and speciation of the paradoxical increase in reactive oxygen species (ROS) production in hypoxia remains debatable. We have developed a series of techniques for measuring superoxide in short times of hypoxia in cells. We have observed that several cell types respond to acute hypoxia with a transient increase in superoxide production for about 10 minutes, probably originated in the mitochondria. This may explain in part the apparently divergent results found by different groups that have not taken into account the time frame of hypoxic ROS production. We propose that cells subjected to hypoxia produce an initial burst of superoxide anion which may be translated in later times into oxidative signals contributing to hypoxic adaptation and preconditioning.
DescriptionResumen del trabajo presentado al X Meeting of the Spanish Group for Research on Free Radicals: "Symposium on Oxidative Stress and Redox Signaling in Biology and Medicine", celebrado en la Facultad de Medicina de la Universidad de Valencia (España) del 2 al 4 de junio de 2014.-- et al.
Appears in Collections:(IIBM) Comunicaciones congresos
(CBM) Comunicaciones congresos
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