English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/125492
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Autophagy is required for apoptotic cell clearance and neural differentiation in early otic development

AuthorsIriarte, Rocío de ; Magariños, Marta ; Rodríguez Aburto, María ; Sánchez-Calderón, Hortensia; Hurlé, Juan M.; Varela-Nieto, Isabel
Issue Date10-Sep-2013
Citation50th Inner Ear Biology Workshop (2013)
AbstractAutophagy is a highly regulated program of self-degradation of the cytosolic constituents that has key roles during early development and in adult cell growth and homeostasis. To investigate the role of autophagy in otic neurogenesis, we studied the expression of autophagy genes in early stages of chicken inner ear development and the consequences of inhibiting the autophagic pathway in organotypic cultures of explanted chicken otic vesicles. Here we show the expression of autophagy-related genes Beclin-1, Atg5 and LC3B during early development of the chicken inner ear. The otic epithelium shows intense lysosomal activity and numerous autophagic vesicles, especially at the neuroblasts exit zone.The inhibition of the transcription of LC3B by using both genetic and pharmacological approaches causes an aberrant morphology of the otic vesicle with accumulation of apoptotic cells. Moreover, inhibition of autophagy provokes the misregulation of the cell cycle in the otic epithelium, impaired neurogenesis and poor axonal outgrowth. Finally, the addition of methyl pyruvate abrogated the consequences of autophagy inhibition. Therefore, our results indicate that autophagy provides the energy required for the clearing of neuroepithelial dying cells and suggest that it is required for the migration of otic neuronal precursors. Taken together, our results show for the first time that autophagy is an active and essential process during early inner ear development.
DescriptionResumen del póster presentado al 50th Inner Ear Biology Workshop, celebrado en Alcala de Henares-Madrid (España) del 10 al 13 de septiembre de 2013.
Appears in Collections:(IIBM) Comunicaciones congresos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.