Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/124835
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Campo DC Valor Lengua/Idioma
dc.contributor.authorSanz, Lorena-
dc.contributor.authorMurillo-Cuesta, Silvia-
dc.contributor.authorCobo, Pedro-
dc.contributor.authorCediel, Rafael-
dc.contributor.authorContreras, Julio-
dc.contributor.authorRivera, Teresa-
dc.contributor.authorVarela-Nieto, Isabel-
dc.contributor.authorAvendaño, Carlos-
dc.date.accessioned2015-11-11T09:32:39Z-
dc.date.available2015-11-11T09:32:39Z-
dc.date.issued2015-
dc.identifierdoi: 10.3389/fnagi.2015.00007-
dc.identifierissn: 1663-4365-
dc.identifier.citationFrontiers in Aging Neuroscience 7: 7 (2015)-
dc.identifier.urihttp://hdl.handle.net/10261/124835-
dc.descriptionThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).-
dc.description.abstractMouse models are key tools for studying cochlear alterations in noise-induced hearing loss and for evaluating new therapies. Stimuli used to induce deafness in mice are usually white and octave band noises that include very low frequencies, considering the large mouse auditory range. We designed different sound stimuli, enriched in frequencies up to 20 kHz (>violet> noises) to examine their impact on hearing thresholds and cochlear cytoarchitecture after short exposure. In addition, we developed a cytocochleogram to quantitatively assess the ensuing structural degeneration and its functional correlation. Finally, we used this mouse model and cochleogram procedure to evaluate the potential therapeutic effect of transforming growth factor β1 inhibitors P17 and P144 on noise induced hearing loss. CBA mice were exposed to violet swept-sine noise with different frequency ranges (2-20 or 9-13 kHz) and levels (105 or 120 dB SPL) for 30 minutes. Mice were evaluated by auditory brainstem response and otoacoustic emission tests prior to and 2, 14 and 28 days after noise exposure. Cochlear pathology was assessed with gross histology; hair cell number was estimated by a stereological counting method. Our results indicate that functional and morphological changes induced by violet swept-sine noise depend on the sound level and frequency composition. Partial hearing recovery followed the exposure to 105 dB SPL, whereas permanent cochlear damage resulted from the exposure to 120 dB SPL. Exposure to 9-13 kHz noise caused an auditory threshold shift in those frequencies that correlated with hair cell loss in the corresponding areas of the cochlea that were spotted on the cytocochleogram. In summary, we present mouse models of noise-induced hearing loss, which depending on the sound properties of the noise, cause different degrees of cochlear damage, and could therefore be used to study molecules which are potential players in hearing loss protection and repair.-
dc.description.sponsorshipThis work was supported by grants from MINECO (SAF2011-24391), Fundación de Investigación Médica Mutua Madrileña (FMM2012), European FP7-INNOVA2-AFHELO and FP7-PEOPLE-IAPP-TARGEAR to IVN and FIS PI 10/00394 for TR. S.M.-C. holds a CIBERER (ISCIII) postdoctoral contract.-
dc.publisherFrontiers Media-
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/304900-
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/612261-
dc.relation.isversionofPublisher's version-
dc.rightsopenAccess-
dc.subjectViolet noise-
dc.subjectCytocochleogram-
dc.subjectHair cells-
dc.subjectHearing loss-
dc.subjectTranstympanic-
dc.subjectTGF-β inhibition-
dc.titleSwept-sine noise-induced damage as a hearing loss model for preclinical assays-
dc.typeartículo-
dc.identifier.doi10.3389/fnagi.2015.00007-
dc.relation.publisherversionhttp://dx.doi.org/10.3389/fnagi.2015.00007-
dc.date.updated2015-11-11T09:32:39Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066eng-
dc.rights.licensehttp://creativecommons.org/licenses/by/4.0/-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
dc.contributor.funderInstituto de Salud Carlos III-
dc.contributor.funderInstituto de Salud Carlos III-
dc.contributor.funderFundación Mutua Madrileña-
dc.contributor.funderEuropean Commission-
dc.contributor.funderCentro de Investigación Biomédica en Red Enfermedades Raras (España)-
dc.relation.csic-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004587es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/100008061es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.pmid25762930-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
Aparece en las colecciones: (IIBM) Artículos
(CETEF) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato
Swept-sine noise-induced.pdf3,3 MBAdobe PDFVista previa
Visualizar/Abrir
Show simple item record

CORE Recommender

PubMed Central
Citations

10
checked on 28-mar-2024

SCOPUSTM   
Citations

11
checked on 20-mar-2024

WEB OF SCIENCETM
Citations

24
checked on 25-feb-2024

Page view(s)

447
checked on 27-mar-2024

Download(s)

359
checked on 27-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


Artículos relacionados:


Este item está licenciado bajo una Licencia Creative Commons Creative Commons