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http://hdl.handle.net/10261/12475
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Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Huerta, Laura | - |
dc.contributor.author | Forment, Javier | - |
dc.contributor.author | Gadea Vacas, José | - |
dc.contributor.author | Fagoaga, Carmen | - |
dc.contributor.author | Peña, Leandro | - |
dc.contributor.author | Pérez-Amador, Miguel A. | - |
dc.contributor.author | García-Martínez, José-Luis | - |
dc.date.accessioned | 2009-04-21T11:54:31Z | - |
dc.date.available | 2009-04-21T11:54:31Z | - |
dc.date.issued | 2008-08-05 | - |
dc.identifier.citation | Plant, Cell & Environment 31(11): 1620-1633 (2008) | en_US |
dc.identifier.issn | 0140-7791 | - |
dc.identifier.uri | http://hdl.handle.net/10261/12475 | - |
dc.description | 14 pages, 4 figures, 4 tables.-- PMID: 18684239 [PubMed].-- Supporting information (Suppl fig. S1, tables S1-S12) available at: http://www3.interscience.wiley.com/journal/121370890/suppinfo | - |
dc.description.abstract | The effect of gibberellins (GA) on internode transcriptome was investigated in transgenic Carrizo citrange (Citrus sinensis × Poncirus trifoliata) plants overexpressing endogenous CcGA20ox1 (encoding a GA biosynthetic gene), and in non-transformed explants treated with GA3, using a citrus cDNA microarray. Substantial modulation of gene expression was found in sense CcGA20ox plants. Extensive up-regulation of genes involved in photosynthesis and carbon utilization, and down-regulation of those involved in protein synthesis and ribosome biogenesis were shown for the first time in plants with higher GA content. Importantly, increase of net photosynthesis in attached leaves was also demonstrated. Expression of other genes belonging to functional groups not reported previously to be regulated by GA (mainly abiotic and biotic stresses, and cuticle biosynthesis), and genes involved in cell division and cell wall architecture were also differentially expressed. Culture of citrus explants for 24 h in GA3 solution produced much lower changes in the transcriptome compared with CcGA20ox plants (1.6% versus 16%, respectively, of total genes in the microarray), suggesting that most of the changes observed in CcGA20ox plants were a consequence of a long-standing GA effect. Interestingly, genes related to abiotic and biotic stresses were similarly modulated in transgenics and GA3-treated explants. | en_US |
dc.description.sponsorship | This work was supported by grants from the Ministerio de Ciencia y Tecnología of Spain (GEN2001-4885-C05). L. Huerta was recipient of a research grant from the Universidad Politécnica de Valencia. | en_US |
dc.format.mimetype | application/octet-stream | - |
dc.language.iso | eng | en_US |
dc.publisher | John Wiley & Sons | en_US |
dc.rights | openAccess | en_US |
dc.subject | Abiotic stress | en_US |
dc.subject | Biotic stress | en_US |
dc.subject | Carbon utilization | en_US |
dc.subject | Microarray | en_US |
dc.subject | Ribosome biogenesis | en_US |
dc.subject | Protein synthesis | en_US |
dc.subject | Transgenics | en_US |
dc.title | Gene expression analysis in citrus reveals the role of gibberellins on photosynthesis and stress | en_US |
dc.type | artículo | en_US |
dc.identifier.doi | 10.1111/j.1365-3040.2008.01870.x | - |
dc.description.peerreviewed | Peer reviewed | en_US |
dc.relation.publisherversion | http://dx.doi.org/10.1111/j.1365-3040.2008.01870.x | - |
dc.identifier.e-issn | 1365-3040 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.openairetype | artículo | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
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HuertaL-Digital.pdf | 406,19 kB | Adobe PDF | Visualizar/Abrir |
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