English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/124674
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


Determination of the DNA conformation of the simian virus 40 (SV40) enhancer in SV40 minichromosomes

AuthorsMartínez-Balbás, Marian ; Casasnovas, José María ; Azorín, Ferran
Issue DateMar-1990
PublisherJohn Wiley & Sons
CitationEuropean Journal of Biochemistry 188(2):269-273 (1990)
AbstractThe simian virus 40 (SV40) enhancer contains three 8-bp purine-pyrimidine alternating sequences which are known to adopt the left-handed Z-DNA conformation in vitro. In this paper, we have undertaken the determination of the DNA conformation adopted by these Z-motifs in the SV40 minichromosome. We have analyzed the presence of Z-DNA through the change in linkage which should accompany formation of this left-handed conformation. Our results indicate that, regardless of the precise moment of the viral lytic cycle at which minichromosomes are harvested and the condition of the transfected DNA, either relaxed or negatively supercoiled, none of the three Z motifs of the SV40 enhancer exist to a significant extent as Z-DNA in SV40 minichromosomes. The SV40 enhancer adopts predominantly a right-handed B-DNA conformation in vivo
Publisher version (URL)http://dx.doi.org/10.1111/j.1432-1033.1990.tb15399.x
Appears in Collections:(IBMB) Artículos
(CID) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.