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dc.contributor.authorTorres-Núñez, Evaes_ES
dc.contributor.authorCal, Lauraes_ES
dc.contributor.authorSuárez-Bregua, Paulaes_ES
dc.contributor.authorGómez-Marín, Carloses_ES
dc.contributor.authorMorán, Palomaes_ES
dc.contributor.authorGómez-Skarmeta, José Luises_ES
dc.contributor.authorRotllant, Josepes_ES
dc.date.accessioned2015-11-05T13:06:52Z-
dc.date.available2015-11-05T13:06:52Z-
dc.date.issued2015-
dc.identifier.citationDevelopmental Dynamics 244(5): 693-702 (2015)es_ES
dc.identifier.issn1058-8388-
dc.identifier.urihttp://hdl.handle.net/10261/124562-
dc.description10 páginas, 5 figuras, 1 tabla.es_ES
dc.description.abstract[Background]: SPARC/osteonectin is an evolutionarily conserved matricellular protein that modulates cell–matrix interaction and cell function. In all vertebrates, SPARC is dynamically expressed during embryogenesis. However, the precise function of SPARC and the regulatory elements required for its expression in particular during early embryogenesis are largely unknown. [Results]: The present study was undertaken to explore the molecular mechanisms that regulate sparc gene expression by in vivo functional characterization of the sparc promoter and identification of possible putative regulatory elements that govern basal promoter activity. We report here transient expression analyses of eGFP expression from transgenic zebrafish containing a Sparc-iTol2-eGFP-BAC and/or 7.25 kb-sparc-Tol2-eGFP constructs. eGFP expression was specifically found in the notochord, otic vesicle, fin fold, intermediate cell mass, and olfactory placode of BAC and Tol2 transposon vectors injected embryos. Deletion analysis revealed that promoter activity resides in the unique 5′-untranslated intronic region. Computer-based analysis revealed a putative CpG island immediately proximal to the translation start site within the intron sequence. Global inhibition of methylation with 5-Aza-2-deoxycytidine promoted sparc expression in association with decreasing CpG methylation. [Conclusions]: Taken together, these data identify a contributory role for DNA methylation in regulating sparc expression in zebrafish embryogenesis.es_ES
dc.description.sponsorshipJ.R. and J.-L.G.-S. were funded by the Spanish Science and Innovation Ministry project. E.T.-N. was supported by a PhD fellowship.es_ES
dc.language.isoenges_ES
dc.publisherJohn Wiley & Sonses_ES
dc.relation.isversionofPostprintes_ES
dc.rightsopenAccessen_EN
dc.subjectSparces_ES
dc.subjectOsteonectines_ES
dc.subjectZebrafishes_ES
dc.subjectTranscriptional regulationes_ES
dc.subjectMethylationes_ES
dc.subjectCpG islandses_ES
dc.titleMatricellular protein SPARC/osteonectin expression is regulated by DNA methylation in its core promoter regiones_ES
dc.typeartículoes_ES
dc.identifier.doi10.1002/dvdy.24267-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1002/dvdy.24267es_ES
dc.identifier.e-issn1097-0177-
dc.embargo.terms2016-03-31es_ES
dc.contributor.funderMinisterio de Ciencia e Innovación (España)-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
dc.relation.csices_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004837es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
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