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Título: | Development and validation of a rapid HPLC method for the quantification of GSE4 peptide in biodegradable PEI-PLGA nanoparticles |
Autor: | Egusquiaguirre, Susana P.; Manguan-García, Cristina CSIC ORCID; Perona Abellón, Rosario CSIC ORCID; Igartua, Manuela | Fecha de publicación: | 2014 | Editor: | Elsevier | Citación: | Journal of Chromatography B 972: 95-101 (2014) | Resumen: | In this work a high performance liquid chromatographic (HPLC) method has been developed and validated for the content determination of GSE4 peptide in PEI-PLGA nanoparticles. Chromatographic separation was performed on a C18 column, and a gradient elution with a mobile phase composed of methanol and 0.1% aqueous trifluoroacetic acid (TFA) solution, at a flow rate of 1ml/min, was used. GSE4 peptide identification was made by fluorescence detection at 290nm. The elution of methanol:TFA was initially maintained at (20:80, v/v) for one min and the gradient changed to (80:20, v/v) in 6min. This ratio was then followed by isocratic elution at (80:20, v/v) during another min and for further 3min it was linearly modified to (20:80, v/v). The developed method was validated according to the ICH guidelines, being specific, linear in the range 10-100μg/ml (R2=0.9996), precise, exhibiting good inter-day and intra-day precision reflected by the relative standard deviation values (less than 3.88%), accurate, with a recovery rate of 100.18±0.95%, and stable for 48h at 5°C or at RT when encapsulated in nanoparticles. The method was simple, fast, and successfully used to determine the peptide content in GSE4-loaded PEI-PLGA nanoparticles. | Descripción: | et al. | URI: | http://hdl.handle.net/10261/124382 | DOI: | 10.1016/j.jchromb.2014.09.041 | Identificadores: | doi: 10.1016/j.jchromb.2014.09.041 issn: 1570-0232 |
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