Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/124340COMPARTIR / EXPORTAR:
 SHARE
 CORE
BASE
|
|
| Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
| Título: | Gasdermin-B promotes invasion and metastasis in breast cancer cells |
Autor: | Hergueta-Redondo, Marta CSIC; Sarrió, David CSIC ORCID; Molina-Crespo, Ángela; Megías, Diego; Mota, Alba CSIC ORCID; Morales, Saleta CSIC; Cano, Amparo CSIC; Moreno-Bueno, Gema CSIC ORCID | Fecha de publicación: | 2014 | Editor: | Public Library of Science | Citación: | PLoS ONE 9(3): e90099 (2014) | Resumen: | Gasdermin B (GSDMB) belongs to the Gasdermin protein family that comprises four members (GSDMA-D). Gasdermin B expression has been detected in some tumor types such as hepatocarcinomas, gastric and cervix cancers; and its overexpression has been related to tumor progression. At least four splicing isoforms of GSDMB have been identified, which may play differential roles in cancer. However, the implication of GSDMB in carcinogenesis and tumor progression is not well understood. Here, we uncover for the first time the functional implication of GSDMB in breast cancer. Our data shows that high levels of GSDMB expression is correlated with reduced survival and increased metastasis in breast cancer patients included in an expression dataset (>1,000 cases). We demonstrate that GSDMB is upregulated in breast carcinomas compared to normal breast tissue, being the isoform 2 (GSDMB-2) the most differentially expressed. In order to evaluate the functional role of GSDMB in breast cancer two GSDMB isoforms were studied (GSDMB-1 and GSDMB-2). The overexpression of both isoforms in the MCF7 breast carcinoma cell line promotes cell motility and invasion, while its silencing in HCC1954 breast carcinoma cells decreases the migratory and invasive phenotype. Importantly, we demonstrate that both isoforms have a differential role on the activation of Rac-1 and Cdc-42 Rho-GTPases. Moreover, our data support that GSMDB-2 induces a pro-tumorigenic and pro-metastatic behavior in mouse xenograft models as compared to GSDMB-1. Finally, we observed that although both GSDMB isoforms interact in vitro with the chaperone Hsp90, only the GSDMB-2 isoform relies on this chaperone for its stability. Taken together, our results provide for the first time evidences that GSDMB-2 induces invasion, tumor progression and metastasis in MCF7 cells and that GSDMB can be considered as a new potential prognostic marker in breast cancer. | Descripción: | This is an open-access article distributed under the terms of the Creative Commons Attribution License.-- et al. | Versión del editor: | http://dx.doi.org/10.1371/journal.pone.0090099 | URI: | http://hdl.handle.net/10261/124340 | DOI: | 10.1371/journal.pone.0090099 | Identificadores: | doi: 10.1371/journal.pone.0090099 issn: 1932-6203 |
| Aparece en las colecciones: | (IIBM) Artículos |
Ficheros en este ítem:
| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| Gasdermin-B.pdf | 12,41 MB | Adobe PDF |  Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
90
checked on 12-abr-2024
SCOPUSTM
Citations
132
checked on 23-abr-2024
WEB OF SCIENCETM
Citations
127
checked on 26-feb-2024
Page view(s)
439
checked on 23-abr-2024
Download(s)
432
checked on 23-abr-2024
